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Shotgun proteomics, FFPE tissue reproducibility

EVALUATION OF CONFIDENCE AND REPRODUCIBILITY OF QUANTITATIVE SHOTGUN PROTEOMIC ANALYSES OF FFPE TISSUES... [Pg.356]

We therefore sought to evaluate reproducibility of shotgun proteomics in studies of archival FFPE tissue. Because FFPE samples are more complex than non-cross-linked samples, we evaluated FFPE human liver for analytical reproducibility and confidence in protein assignments.20 This complexity strengthens the argument for using high-resolution separations to maximize analyte concentration and minimize matrix effects. In this case, we used transient capillary isotachophoresis/capillary zone electrophoresis (cITP/cZE) in place of IEF to help address this effect. cITP/cZE has a resolution superior even to cIEF (90% of identified peptides in 1 fraction, 95% in 2 fractions or less for cITP/cZE, vs. 75% and 80%, respectively, for cIEF). [Pg.356]

See other pages where Shotgun proteomics, FFPE tissue reproducibility is mentioned: [Pg.347]    [Pg.347]   
See also in sourсe #XX -- [ Pg.354 , Pg.355 , Pg.356 , Pg.357 , Pg.358 ]

See also in sourсe #XX -- [ Pg.354 , Pg.355 , Pg.356 , Pg.357 , Pg.358 ]



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Reproducibility

Reproducible

Shotgun proteomics

Shotgun proteomics, FFPE tissue

Tissue FFPE)

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