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Shotgun proteomics, FFPE tissue

THE PROMISE AND CHALLENGE OF SHOTGUN PROTEOMICS IN ARCHIVAL, FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUES (FFPE)... [Pg.347]

EVALUATION OF CONFIDENCE AND REPRODUCIBILITY OF QUANTITATIVE SHOTGUN PROTEOMIC ANALYSES OF FFPE TISSUES... [Pg.356]

We therefore sought to evaluate reproducibility of shotgun proteomics in studies of archival FFPE tissue. Because FFPE samples are more complex than non-cross-linked samples, we evaluated FFPE human liver for analytical reproducibility and confidence in protein assignments.20 This complexity strengthens the argument for using high-resolution separations to maximize analyte concentration and minimize matrix effects. In this case, we used transient capillary isotachophoresis/capillary zone electrophoresis (cITP/cZE) in place of IEF to help address this effect. cITP/cZE has a resolution superior even to cIEF (90% of identified peptides in 1 fraction, 95% in 2 fractions or less for cITP/cZE, vs. 75% and 80%, respectively, for cIEF). [Pg.356]

Archival tissues stored as FFPE tissue blocks represent the vast majority of tissue archives. To investigate the effect of storage on shotgun proteomic analysis of FFPE tissues, we analyzed 10 samples dating back to 1980.22 Nine leiomyomas (three from 1990, three from 1997, and three from 2002) and one... [Pg.359]

This study demonstrated that even decades-old archival FFPE tissues are amenable to shotgun proteomic profiling and yield evaluable results. [Pg.361]

SHOTGUN PROTEOMICS FOR THE ANALYSIS OF ARCHIVAL FFPE TISSUES 359... [Pg.359]

FUTURE DIRECTIONS FOR SHOTGUN PROTEOMICS APPLIED TO FFPE TISSUES 361... [Pg.361]

See other pages where Shotgun proteomics, FFPE tissue is mentioned: [Pg.347]    [Pg.348]    [Pg.348]    [Pg.348]    [Pg.349]    [Pg.349]    [Pg.350]    [Pg.352]    [Pg.358]    [Pg.359]    [Pg.364]    [Pg.366]    [Pg.347]    [Pg.348]    [Pg.348]    [Pg.348]    [Pg.348]    [Pg.349]    [Pg.349]    [Pg.350]    [Pg.350]    [Pg.352]    [Pg.354]    [Pg.356]    [Pg.358]    [Pg.359]    [Pg.360]    [Pg.362]   
See also in sourсe #XX -- [ Pg.347 , Pg.348 , Pg.349 , Pg.350 , Pg.351 , Pg.352 , Pg.353 , Pg.354 , Pg.355 , Pg.356 , Pg.357 , Pg.358 , Pg.359 , Pg.360 , Pg.361 , Pg.362 , Pg.363 ]

See also in sourсe #XX -- [ Pg.347 , Pg.348 , Pg.349 , Pg.350 , Pg.351 , Pg.352 , Pg.353 , Pg.354 , Pg.355 , Pg.356 , Pg.357 , Pg.358 , Pg.359 , Pg.360 , Pg.361 , Pg.362 , Pg.363 ]



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Proteomic analysis shotgun proteomics, FFPE tissue

Shotgun proteomics

Shotgun proteomics, FFPE tissue reproducibility

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