Serum albumin effect

Whitley, R. D., Wachter, R., Liu, F., and Wang, N.-H. L (1989). Ion-exchange equilibria of lysozyme, myoglobin and bovine serum albumin. Effective valence and exchanger capacity. J. Chromatogr. 465, 137-156. 

McMenamy, R. H. The binding of indole analogues to human serum albumin Effects of fatty acids, /. Biol. Chem., 240, 4235, 1965. 

H. Edelhoch, R. LoNTiEand P. R. Morrison Light Scattering in Solutions of Serum Albumin Effects of Charge and Ionic Strength. J. Amer. chem. Soc., 72, 4641—4656 (1950). 

Achiral manganese porphyrins Mn(TPP) and (TPP)Cl included into a carrier protein (bovine serum albumin) effect the stereoselective dioxygenation of L(+)- and D(-)-AA [228]. 

Sulfaphenazole (684) and sulfazamet (685) are both examples of relatively short acting sulfonamides (B-80MI40406) and their antibacterial activity has been tested against Escherichia coli, the former being more effective than the latter. Sulfaphenazole also displaces sulfonyl ureas from protein binding sites on human serum albumin and consequently increases the concentration of the free (active) drug and produces a more intense reaction that may result in hypoglycemia. 

The effect of flow rate on resolution by Toyopearl HW-55F and Toyopearl HW-55S columns has been studied using a bovine serum albumin sample. Eor both columns, resolution decreased with increasing flow rate (46). Resolution is increased, however, with decreasing particle size (47). Resolution is proportional to the square root of the column length, as theoretically expected, and indicates that longer columns can be packed as well as shorter columns. Therefore, for samples difficult to resolve, the solution may be to increase the column length. 

The qualitative thermodynamic explanation of the shielding effect produced by the bound neutral water-soluble polymers was summarized by Andrade et al. [2] who studied the interaction of blood with polyethylene oxide (PEO) attached to the surfaces of solids. According to their concept, one possible component of the passivity may be the low interfacial free energy (ysl) of water-soluble polymers and their gels. As estimated by Matsunaga and Ikada [3], it is 3.7 and 3.1 mJ/m2 for cellulose and polyvinylalcohol whereas 52.6 and 41.9 mJ/m2 for polyethylene and Nylon 11, respectively. Ikada et al. [4] also found that adsorption of serum albumin increases dramatically with the increase of interfacial free energy of the polymer contacting the protein solution. 

Andry, M. C. Levy, M. C. (1997). In vitro degradation of serum albumin microcapsules Effect of process variables. International Journal of Pharmaceutics, Vol. 152, 2, (Jime 1997), pp. (145-151), ISSN 0378-5173... 

Edwards-Levy, F., Andry, M. C. Levy, M. C. (1994). Determination of free amino group content of serum-albumin microcapsules. II. Effect of variations in reaction-time and terephthaloyl chloride concentration. International Journal of Pharmaceutics, Vol. 103, 3, (March 1994), pp. (253-257), ISSN 0378-5173 Friend, D. R. (2005). New oral delivery systems for treatment of inflammatory bowel disease. Advanced Drug Delivery Reviews, Vol. 57, 2, (January 2005), pp. (247-265), ISSN 0169-409X... 

Miyake et al reported an ELISA method for the determination of pesticide residues in the aquatic environment. The polyclonal antibody and three monoclonal antibodies of acifluorfen were prepared by immunization of rabbits and mice with acifluorfen-bovine serum albumin conjugates. The polyclonal antibody reacted with acifluorfen at concentrations of 1.5-800 mg L , while the monoclonal antibodies reacted with acifluorfen at concentrations of 1.5-144 mg L . Among three monoclonal antibodies, AF 75-144 reacted with chlornitrofen, which did not react with the other two antibodies. It seems that the ELISA method is effective for the determination of herbicide residues in the aquatic environment. 

Figure 34 Effective cell partition coefficient of PNU-78,517 as a function of bovine serum albumin concentration. The inset shows the relationship between the effective permeability coefficient (Pe) of appearance and BSA concentration or the fraction of free drug. These data were obtained from the uptake data shown in Figure 33. [Redrawn from Raub et al. (1993) with permission from the publisher.]...

Fast and simple 2D HPLC was also shown to be effective for the separation of a tryptic digest of bovine serum albumin (BSA) (Kimura et al., 2004). Every... 

Daniel E, Weber G (1966) Cooperative effects in binding by bovine serum albumin. I. The binding of l-anilino-8-naphthalenesulfonate. Fluorimetric titrations. Biochemistry 5 1893-1900... 

Figure 6 Effect of the increased rotational correlation time on the proton relaxivity of MP2269, a Gd111 chelate capable of noncovalent protein binding (Scheme 2). The lower NMRD curve was measured in water, whereas the upper curve was obtained in a 10%w/v bovine serum albumin solution in which the chelate is completely bound to the protein. The rotational correlation times calculated are rR=105ps in the nonbound state, and rR= 1,000 ps in the protein-bound state (t=35°C). For this chelate, the water exchange...

The effect of the hydrophobicity of the paramagnetic complex on the affinity towards serum albumin has been studied by comparing the K l values for a series of Gd111 complexes bearing an... 

Flora, K.K. and Brennan, J.D. (2001) Effect of matrix aging on the behavior ofhuman serum albumin entrapped in a tetraethyl orthosilicate-derived glass. Chemistry of Materials, 13, 4170—4179. 

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