Serum albumin, bovine buffering requirements

Protein molecular weight standards—Suggested standards are phosphorylase (97,400 Da), bovine serum albumin (66,200 Da), ovalbumin (45,000 Da), carbonic anhydrase (31,000 Da), soybean trypsin inhibitor (21,500 Da) and lysozyme (14,400 Da) at 1 mg/ml each in a single mixture in 0.01 M Tris chloride buffer, pH 7.0 (prepared by dilution of the stock 1 M buffer above). Approximately 2 ml of this solution will be required. 

Blocking buffer is PBS containing a final concentration of 1% bovine serum albumin (BSA) and 0.05% Tween-20. This should be made in volumes necessary to complete tests as required, but can be stored at 4jaC if contamination is avoided, and should always be warmed and inspected for contamination before use. 

Oxone sulfoxidations can show appreciable diastereoselectiv-ity in appropriate cases, as demonstrated in eq 26. Enantios-elective oxidations of sulfides to sulfoxides have been achieved by buffered aqueous Oxone solutions containing bovine serum albumin (BSA) as a chiral mediator (eq 27). As little as 0.05 equiv of BSA is required and its presence discourages further oxidation of the sulfoxide to the sulfone. Oxone can be the active oxidant or reaction can be performed in the presence of acetone. 

Chitosan was also formulated in a controlled-release protein delivery system using bovine serum albumin (BSA) as a model drug. Chitosan was reacted with sodium alginate in the presence of tripolyphosphate for bead formation [94]. Parenteral administration of proteins/peptides requires repeated injections because of their extremely short biological half-life. Daily multiple injections are highly risky and require close medical supervision. On this basis, bovine serum albumin (BSA)-loaded chitosan microspheres were prepared to test the drug release behavior in buffers with different pH values. BSA-chitosan microspheres with particle sizes in the range from 5 to 10 pm... 

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