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Sequences study techniques

IR-spectroscopy technique has been used meaningfully in the qualitative reaction sequence studies... [Pg.334]

Fraenkel-Conrat, H., J. I. Harris, and A. L. Levy Recent developments in techniques for terminal and sequence studies in pe])tides and proteins. In. .Methods of Biochemical Analysis", Vol. 2, 359—425 Ed. D. Click, Interscience Publ., 1955. [Pg.35]

Tire MALDI method is especially useful for complex mixtures of peptides and can be utilized in peptide sequencing. The technique is also appropriate for studying mixtures of glycoproteins. Negative-ion MALDI can be applied to oligonucleotide mixtures. Further improvements in resolution in both MALDI... [Pg.113]

Choice of Probes. The sequence chosen for analysis will be determined by the nature of the evolutionary comparison to be carried out. Examples of probe DNAs used for examining populational and species level comparisons, as well as for higher level systematics, are described by Dowling et a/.38 Table II provides some of die cloned sequences (or techniques to obtain them)45-75 that have the potential to be useful in evolutionary studies. The list is not meant to be comprehensive but representative there... [Pg.163]

Fraenkel-Conrat, H., Harris,]. leuan, and Levy, A. L, Recent Developments in Techniques for Terminal and Sequence Studies in Peptides and... [Pg.239]

Peptides, Terminal and Sequence Studies in. Recent Developments in Techniques for... [Pg.257]

H- and 13C-NMR data have been reported for diagnostic purposes in direct analysis of phenylthiohydantoin amino acid derivatives (PTH) produced in the Edman degradation of peptides and proteins.189-193 The insensitivity of 3H-NMR spectroscopy constitutes a major hurdle for its application in the sequence study of peptides.194,195 Alternatively, identification of the cleaved amino acids in the automated Edman degradation has been solved in some cases by using IR,196-198 mass,199 and gas chromatographic techniques.200... [Pg.207]

So far in this chapter on sequencing, two techniques have predominated NMR spectroscopy and Py-GC. An exception is the work of Zhuang and co-workers [67] who used ToF-SIMS in their study of the distribution of polydimethylsiloxane (PDMS) segment lengths at the surface of PDMS-urethane-segmented copolymers. Their aim was to establish whether, at the copolymer surface, the distribution of segment or chain lengths is different from that in the bulk of the polymer. [Pg.199]


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See also in sourсe #XX -- [ Pg.212 , Pg.213 , Pg.214 , Pg.218 ]




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