Sequence coverage, complete

The systematic characterization of secondary protein modifications is different from sequencing a protein. For a systematic analysis of protein modifications, the complete protein sequence should be covered by the investigation. This is difficult to achieve since the enzymatic digestion of a protein is not homogeneous. The sequence coverage depends on the nature of the protein, its quantity, and the enzyme used. Typical... 

It is unusual for either CID or ETD to provide complete sequence information from any one peptide but the use of both techniques provides complementary information, which can gready extend the sequence coverage (Table 4). In addition, because the energy from the ETD process is directed into cleaving the Ca—N bond, the labile PTMs are preserved and their location in the peptide sequence can then be determined90 (see also discussion in Section 9.10.3.2.6 on the use of CID/IRMPD and ECD/ETD for protein/peptide sequencing, and Table 4). 

Sequence coverage needed to achieve 95% of complete sequence 1 10-15... 

Sequence coverage needed to compare two genomes and isolate clones/genes completely covering regions of divergence 4% (0.04-fold) At least 6-8-fold (600%-800%)... 

ESI-Infrared Multiphoton Dissociation IRMPD of ESI-generated ions can also provide sequence information. By combining the data from the nozzle-skimmer dissociation and IRMPD experiments, nearly complete sequence coverage for larger oligonucleotides can be obtained [48]. These two dissociation techniques yield complementary sequence ions. For example, IRMPD primarily produces w- and (a — B )-type sequence ions, whereas the nozzle-skimmer dissociation generates b-, c-, and [Pg.467]

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