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Sequence-based screening methods

Besides novel nticioorganisms isolation and culturing, eDNA library directly extracted from extremely complicated circumstance, especially in untouched terrestrial or marine source is also an invaluable treasure for antibiotics discovery (Banik and Brady 2010 Li and (Jin 2005). Currently, heterologous expression of eDNA clones coupling with biological activity or sequence-based screening methods were... [Pg.107]

The biological methods of preparation of peptide libraries were introduced by three different groups in 1990 [4-6], These methods, which are also based on combinatorial principles, can be used to prepare even billions of peptide sequences. These sequences appear attached to the end of protein chains. Only the natural L-amino acids can be used as monomers in composition of the libraries. Very effective screening methods are also developed to determine potential bioactive sequences. [Pg.9]


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See also in sourсe #XX -- [ Pg.123 ]




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Base Sequence

Based Screens

Method screening

Screening-Methode

Sequencing methods

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