Sequence analysis of short DNA fragments

The initial requirement is for a singly end-labelled DNA fragment or a uniformly labelled single-strand. If the fragment molecule is single-stranded e.g. a chemically synthesized primer, all that 

Detailed procedures for 5 - or 3 -end labelling DNA fragments, following the method of Maxam and Gilbert (1980) are given in Chapter 5. Essentially similar protocols are given by Wu et al. (1976) and Roychoudhury and Wu (1980). 

Long duplex DNA fragments are resolved into their complementary single-strands by denaturation followed by electrophoresis on a 5-8% acrylamide gel (Maxam and Gilbert, 1980 this volume, Chapter 5). 

Smaller fragments ( 40 nucleotides) are resolved into single- 

Deduction of complete sequence through overlap in the middle 

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