Separator Acclaim Trinity

In pharmaceutical formulations, the most commonly used counterions are sodium and chloride ions. While no other reversed-phase column can retain or separate them, the Acclaim Trinity PI column overcomes this challenge by providing excellent separations and peak shapes for both analytes (see Figure 6.89). However, this requires a nonspecific detection method, because both ions are nonchromophoric and cannot be detected simultaneously by suppressed... 

Figure 6.89 Simultaneous separation of sodium and chloride ions on Acclaim Trinity PI, 3 (im. Column dimensions 50 mm x 3 mm i.d. column temperature 30 °C eluent MeCN/ 15 mmol/L ammonium acetate, pH 5.2 (60 40...

Figure 6.90 Simultaneous isocratic separation of pharmaceutical counterions on Acclaim Trinity PI, 3 pm. Column dimensions 50mm X3mm i.d. column temperature 30°C eluent MeCN/20 mmol/L ammonium acetate, pH 5 (60 40 v/v) flow rate 0.5 mL/min detection ...

Figure 6.93 shows the fast separation of paraquat and diquat on the 50mmx 2.1 mm i.d. Acclaim Trinity Ql using a mobile phase of ammonium acetate and... 

Figure 6.93 Fast separation of paraquat and ammonium acetate, pH 5 (75 25 v/v) flow diquat on Acclaim Trinity Ql, 3 pm. Column rate 0.5 mtymin detection UV (290 nm) injec-dimensions 50 mm x 2.1 mm i.d. column tern- tion volume 10 pL peaks 10 mg/L each of perature 30 °C eluent MeCN/100 mmol/L paraquat (1) and diquat (2).

Figure 6.96 Separation of mono- and multivalent pharmaceutical counterions on the 3 pm Acclaim Trinity PI column. Column dimensions 50 mm X 2.1 mm i.d. column temperature 30 °C eluent ammonium acetate, pH 3.65 gradient lOmmol/L for 0-1 min and then linearly to 100 mmol/L in 10 min flow...

Figure 7.9 Comparison of the chromatographic behavior of pharmaceuticai counterions between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns lOOmmxSmm i.d. Acclaim Trinity PI, 3 pm, and 150mmx4.6 mm i.d. ZIC-HILIC, 5 pm coiumn temperature 30 °C eiuent (A) 60 40 (v/v) MeCN/0.02 moi/L NH4OAC, pH 5 and eiuent (B) 80 20 (v/v) MeCN/0.015 moi/L NH4OAC, pH 5 flow rates ...

Figure 7.11 Comparison of the chromatographic behavior of a hydrophobic acidic API and its counterion between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns ...

Figure 7.22 Separation of penicillin G on Acclaim Trinity PI under RP and HILIC conditions. Column dimensions SOmmxSmm i.d., 3 urn column temperature SOT eluent ...

Analogously, Figure 6.88 in Section 6.7.2 illustrated the separation of 1,1-dimethylbiguanide (Metformin) and its chloride counterion at four different acetonitrile levels on Acclaim Trinity PI. 1,1-Dimethylbiguanide hydrochloride is a highly hydrophilic, basic antidiabetic drug that reduces blood glucose levels and improves insulin sensitivity. Due to the hydrophilic nature of the analytes and... 

Figure 7.24 Separation of seiected Good s buffers on Acclaim Trinity PI under HILIC conditions. Column dimensions lOOmmxSmm i.d., 3 pm column temperature 30 °C eluent 85 15 (v/v) MeCN/12 mmol/L ammonium...

Figure 8.65 Simultaneous separation of a basic drug and its counterion utilizing UV and ELS detectors in series. Separator column Acclaim Trinity PI, 3 pm column dimensions 50 mm X3 mm i.d. column temperature 30°C eluent 20 80 (v/v) MeCN/30 mmol/L...

Figure 8.77 Simultaneous measurement of (a) diclofenac and its sodium counterion, and (b) chloride impurity. Separator column Acclaim Trinity PI, 3pm column dimensions 50mm X 3 mm i.d. column temperature 30 °C eluent 75 25 (v/v) MeCN/200 mmol/L ammonium...

Figure 8.117 LC-MS for simultaneous analysis of ionic liquids, counterions, and impurities. Separator column Acclaim Trinity PI, 3 pm column dimensions 100mmx2.1 mm i.d. eluent MeCN/5 mmol/L NH4OAC, pH 5.2 gradient 55% MeCN (v/v) for 0-2 min, to 60% (v/v) in 8 min, and then to 90% (v/v) in 1 min and isocratic for 7min flow rate 0.4mL/min detection MS (Thermo Scientific MSQ Plus) source voltage 1 kV needle temperature ...

With the introduction of Acclaim Trinity PI, a broad range of hydrophobic and hydrophilic APIs that possess either acidic or basic functional groups can be separated along with their respective positive or negative counterions. As an example, the separation of the hydrophilic and basic API, 1,1-dimethylbiguanide (metformin), from its chloride counterion can be achieved using a mobile phase with a relatively low amount of acetonitrile (20% v/v) under isocratic conditions. 

Metformin is an oral antidiabetic drug to treat diabetes mellitus type 2 it is believed to be the most widely prescribed antidiabetic drug in the world. Figure 10.307 shows the separation of metformin and its chloride counterion on Acclaim Trinity PI under isocratic conditions. 

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