Acclaim Trinity

Figure 6.86 Schematic illustration of the Acclaim Trinity PI packing material based on NSH technology.

Figure 6.87 Selectivity adjustment by changing (a) ionic strength and (b) pH in the mobile phase on Acclaim Trinity PI, 3 pm. Column dimensions 50 mm x 3 mm i.d. column temperature 30 °C eluent (A) MeCN/ammonium...

In pharmaceutical formulations, the most commonly used counterions are sodium and chloride ions. While no other reversed-phase column can retain or separate them, the Acclaim Trinity PI column overcomes this challenge by providing excellent separations and peak shapes for both analytes (see Figure 6.89). However, this requires a nonspecific detection method, because both ions are nonchromophoric and cannot be detected simultaneously by suppressed... 

Figure 6.90 Simultaneous isocratic separation of pharmaceutical counterions on Acclaim Trinity PI, 3 pm. Column dimensions 50mm X3mm i.d. column temperature 30°C eluent MeCN/20 mmol/L ammonium acetate, pH 5 (60 40 v/v) flow rate 0.5 mL/min detection ...

Figure 6.93 shows the fast separation of paraquat and diquat on the 50mmx 2.1 mm i.d. Acclaim Trinity Ql using a mobile phase of ammonium acetate and... 

Figure 6.93 Fast separation of paraquat and ammonium acetate, pH 5 (75 25 v/v) flow diquat on Acclaim Trinity Ql, 3 pm. Column rate 0.5 mtymin detection UV (290 nm) injec-dimensions 50 mm x 2.1 mm i.d. column tern- tion volume 10 pL peaks 10 mg/L each of perature 30 °C eluent MeCN/100 mmol/L paraquat (1) and diquat (2).

To Study the retention behavior of both Acclaim Trinity columns in the HILIC mode, three highly hydrophilic compounds with different charge states can be used as test probes weso-erithritol (neutral), Tris (cationic), and glyceric acid (anionic). 

Figure 6.96 Separation of mono- and multivalent pharmaceutical counterions on the 3 pm Acclaim Trinity PI column. Column dimensions 50 mm X 2.1 mm i.d. column temperature 30 °C eluent ammonium acetate, pH 3.65 gradient lOmmol/L for 0-1 min and then linearly to 100 mmol/L in 10 min flow...

The four Acclaim Mixed-Mode columns mentioned in Section 6.7.2 - Acclaim Mixed-Mode WAX-1, Acclaim Mixed-Mode WCX-1, Acclaim Trinity PI, and Acclaim Trinity P2 - can also be used under HILIC conditions. Acclaim Mixed-Mode WAX-1 and Acclaim Mixed-Mode WCX-1 are based on an alkyl chain with terminal ion-exchange functional groups. Because of this column chemistry, both columns offer multiple retention mechanisms, including ion exchange, reversed phase, and HILIC. As an example. Figure 7.7 demonstrates that in highly organic mobile phase. Acclaim Mixed-Mode WCX-1 can serve as a HILIC column. Hydrophilic analytes such as cytosine elute later than naphthalene as a hydrophobic neutral analyte. 

Figure 7.9 Comparison of the chromatographic behavior of pharmaceuticai counterions between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns lOOmmxSmm i.d. Acclaim Trinity PI, 3 pm, and 150mmx4.6 mm i.d. ZIC-HILIC, 5 pm coiumn temperature 30 °C eiuent (A) 60 40 (v/v) MeCN/0.02 moi/L NH4OAC, pH 5 and eiuent (B) 80 20 (v/v) MeCN/0.015 moi/L NH4OAC, pH 5 flow rates ...

Figure 7.11 Comparison of the chromatographic behavior of a hydrophobic acidic API and its counterion between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns ...

Figure 7.22 Separation of penicillin G on Acclaim Trinity PI under RP and HILIC conditions. Column dimensions SOmmxSmm i.d., 3 urn column temperature SOT eluent ...

Analogously, Figure 6.88 in Section 6.7.2 illustrated the separation of 1,1-dimethylbiguanide (Metformin) and its chloride counterion at four different acetonitrile levels on Acclaim Trinity PI. 1,1-Dimethylbiguanide hydrochloride is a highly hydrophilic, basic antidiabetic drug that reduces blood glucose levels and improves insulin sensitivity. Due to the hydrophilic nature of the analytes and... 

Figure 7.24 Separation of seiected Good s buffers on Acclaim Trinity PI under HILIC conditions. Column dimensions lOOmmxSmm i.d., 3 pm column temperature 30 °C eluent 85 15 (v/v) MeCN/12 mmol/L ammonium...

Figure 8.65 Simultaneous separation of a basic drug and its counterion utilizing UV and ELS detectors in series. Separator column Acclaim Trinity PI, 3 pm column dimensions 50 mm X3 mm i.d. column temperature 30°C eluent 20 80 (v/v) MeCN/30 mmol/L...

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