Separation techniques and mass spectrometry

But are there other endogenous substrates for this enzyme A method based on separation techniques and mass spectrometry has been developed to identify endogenous substrates of enzymes by analysis of metabolomes from wild-type and enzyme-inactivated organisms [316], Indeed, the accumulation of metabolites resulting from the inactivation of the enzyme would be considered candidate endogenous substrates for this enzyme. This method based on comparative metabolomics is called discovery metabolites profiling (DMP). 

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