Separation practical hints

Give exact practical details of the proposed procedure (use separate sheets if necessary). You should include any practical tips/hints gained through personal experience. 

Too many chromatographic texts dwell heavily on a theoretical and mathematical complexity that bears little relevance to what you actually need to do in order to practice preparative HPLC. Hopefully this book will describe how to practically go about a preparative separation. It is designed to guide the reader through the choice of equipment and chromatographic modes with minimal fuss and with reference to only relevant formulae. Much of the black art will be removed by the hints and tips of a practitioner with over 20 year s experience in many modes of chromatographic separation. 

This possible interaction is fairly well documented, but the results are conflicting. In practice it appears not to be important in most patients, although some loss of seizure control has been seen to occur in isolated cases. The interaction is unpredictable because it seems to depend on the individual patient and the antacid being taken. Concurrent use need not be avoided but if there is any hint that phenytoin levels are reduced, separation of the dosages by 2 to 3 hours may, as with other interactions caused by antacids, minimise the effects. 

Peel transfer Once the slurry has been filtered, carefully separate the vacuum apparatus, take off the filter, and transfer the clay film from the filter to a standard glass petrographic slide. This is usually the most difficult task of the preparation procedure and requires considerable practice. Refer to Reynolds Moore (1997) and Drever (1973) for hints and guidelines on making successful peel transfers. 

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