Separation of Lipophilic, Steam-volatile Mixtures

It is frequently necessary to concentrate the terpenes and their derivatives in a reaction charge or in plant or animal material before separating them. As mentioned already in the introduction, the C5- to Ci5-compounds which come into consideration are volatile in steam and can thus be isolated, using appropriate apparatus [81, 174]. The Karlsruhe apparatus (Firm 21) has proved the best for quantitatively isolating small amounts (1—1000 mg) of such mixtures (Fig. 101). The lipophilic fraction which has distilled over can be transferred, free of water, to a small microflask for weighing it can then be investigated according to the scheme in Table 22. 

About 300—400 mg oil are needed for determination of density according to Furter [67 a], refractive index with the Abbe-refractometer 

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