Sensitized fluorescence, first demonstrated

Applications. The general feasibility of CE to separate immunocomplex from unbound reagent was first demonstrated by Grossman et al. [4] and Nielsen et al. [23], whose reports set the stage for further development of quantification techniques in CE-IA. The first quantitative noncompetitive CE assay of insulin was reported by Schultz and Kennedy [24], To increase the sensitivity of analysis, the authors used FITC-labeled insulin and LIF detection. Due to the separation power of CE, multiple products of fluorescence labeling of insulin did not hamper analysis (see Figure 6). Two types of calibration curves were con-... 

Fluorescent SAMs on glass have been developed by Crego-Calama et al. as new sensor materials. In 1999, the phenomenon of proximal but spatially separable receptor-fluorophore communication was recorded in solution by Tonellato et al.33 and in 2001 Crego et al. demonstrated for the first time that disconnection between fluorophore and receptor can be applied to the preparation of stable sensitive fluorescent materials for metal ion sensing.34 They used SAMs on glass substrates as a 2D scaffold to impart sufficient molecular orientation to separately deposit various binding functionalities (rather than the entire receptor molecule) and the fluorophore on the surface to achieve analyte selectivity. This approach avoids the synthesis and... 

Direct information on excitation transfer is obtained from sensitization experiments in which the transfer results in the replacement of donor-emission by acceptor-emission. The first demonstration of fluorescence sensitization was achieved with atomic vapours by Cario and Franck in 1922. Some years later, Perrin and Choucroun (1927) reported similar experiments on singlet-singlet transfer in solution by the process... 

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