Semi-synthetic peroxidase

A similiar approach was performed by van de Velde (1999), using incorporation of vanadate into an acid phosphatase (phytase) to create a semi-synthetic peroxidase similar to the heme-dependent chloroperoxidase. The latter is a useful enzyme for the asymmetric epoxidation of olefins, but less stable due to oxidation of the porphyrin ring and difficult to express outside the native fungal host. The authors exploited the structural similarity of active sites from vanadate-dependent halo-peroxidases and acid phosphatases and have shown the useful application as an enantioselective catalyst for the synthesis of chiral sulfoxides (van de Velde, 1999). 

D Haring, P Schreier. Novel biocatalysts by chemical modification of known enzymes cross-linked microcrystals of semi-synthetic peroxidase seleno-subtilisin. Angew Chem Int Ed 37 2471-2473, 1998. 

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