Selectivity for Influenza Virus Sialidase over Human Sialidases

4 Selectivity for Influenza Virus Sialidase over Human Sialidases 

Neither 4-amino- nor 4-guanidino-4-deoxy-Neu5Ac2en showed cytotoxicity against human cell lines at concentrations up to 10 mM [121]. Due to their charge, the compounds have poor capacity to cross membranes, and a study of radiolabelled 4-guanidino derivative 5 showed that it did not enter either influenza virus-infected or uninfected MDCK cells [122]. 

It was important to determine the effect of the 4-substituted Neu5Ac2en derivatives on endogenous human sialidases. Four human sialidases (Neul-4) have been identified to date (reviewed in [103,104]). Of these, Neu3 is predomi-nantiy localized in the plasma membrane, and both Neul and Neu4 have been 

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