Selection of the Stationary Phase and Method

When the list of reactants, cofactors, and reaction conditions has been compiled, the stationary phase can be selected. 

To select the appropriate stationary phase, it is necessary to examine the reactants to determine how they differ. The selection of the stationary phase should exploit this difference. For example, do the reactants differ in size, charge, or solubility Examination of the modes of operation of the stationary phase materials presented in Chapter 2 reveals gel filtration to be ideal for 

4 Modification of Reaction Conditions for the HPLC Assay Method 

The importance of understanding secondary reactions cannot be overemphasized. This knowledge is invaluable to the interpretation of chromatographic 

These chromatograms were studied with the expectation that the enzyme was pure. Therefore, we thought the chromatogram would show only the 

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