Screening phenotype

Mutagenic PGR. More recently, methods have been developed to use the PGR reaction to randomly mutagenize a defined sequence (25). The Taq polymerase used in PGR misincorporates nucleotides in a random fashion if manganese dichloride [7773-01 -5] MnGl2, is included in the reaction buffer during PGR. The Hbrary of mutagenized PGR products can be screened for the desired phenotype. 

Mayer TU, Kapoor TM, Haggarty SJ et al (1999) Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 286 971... 

Hart CP. Finding the target after screening the phenotype. Drug Discov Today 2005 10 513-9. 

Screening for phenotype rather than mechanism was the norm in the 1970s, an era at least as productive as the current in terms of drug approvals [12]. Screening for mechanism predominated by the 1990s and still does today. Most drugs entering... 

Phenotypic screens seek compounds with a specific effect on cell physiology, by adding compounds to living cells, and scoring for an effect. The measurable... 

Kogma Did your screen uncover other genes with a similar phenotype ... 

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