Screening method performance requirements

Apart from confirmatory analytical methods, quality criteria have been also defined for screening tests. Screening tests must be demonstrated as having been performed with acceptable figures of merit for accuracy, specificity, and reproducibility. Although no esoteric performance requirements are peculiar to screening tests, some particular points are especially useful in tire evaluation of screening tests. 

Ligand-detected NMR is a robust method, which is well suited to screening compound mixtures with rapid deconvolution [69], The target protein does not have to be labeled and its size is typically much more than 20 kDa. The protein production requirements are considered to be moderate. If the screen is performed in the presence of a competitor with known interaction mode, active site versus nonactive site binders can be distinguished and binding affinities can be derived. 

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