Schizophyllum commune, enzyme

En me Treatment of LCCs. Purified Bacillus circulans xylanase (specific activity, 110 units/mg protein), purified Coriolus versicolor )9-mannanase (specific activity 179 units/mg protein), purified Thermoascus aurianticum /8-glucosidase (specific activity, 150 units/mg protein), and purified Schizophyllum commune acetyl xylan esterase (AXE specific activity, 180 units/mg protein) were used. Unit activity for xylanase and )9-mannanase was defined as the amount of enzyme catalyzing the release of 1 /xmol reducing power per minute at 50°C and pH 6.0 using oat spelts... 

An endo-1,4-j8-xylanase (xylanase A), obtained from Schizophyllum commune, is rapidly deactivated using l-(4-azonia-4,4-dimethylpentyl)-3-ethylcarbodiimide iodide, indicating that the reactive site in the enzyme is a carboxyl group. " Xylanase A is of importance for the conversion of hemicellulosic biomass into fermentable products. 

Desulfation of organic sulfate esters with the release of sulfate ion is identified with enzymes such as glycosulfatase in the red algae, Porphyra umbilicus (Rees, 1961). However organic esters may be reduced to liberate methanethiol or dimethyl sulfide. Young and Maw (1958) reported that the fungus, Schizophyllum commune, synthesises dimethyl sulfide and methyl sulfide by the following successive reduction and methylation reactions ... 

Another source of the metal ion content of lignin is from the enzyme solutions. Two types of enzymatic preparations were used a mixture of the cellulases produced by cultures of Schizophyllum commune and Trichoderma reesei ( ) and a commercial preparation (Cellucast, Novo industry, Denmark) which is a concentrate of the cellulases produced by Trichoderma viride. Both types of enzyme preparation contain metal ions derived from the culture media. 

C20H30O4, Mr 334.46, cryst., mp. 119-122°C. A diterpenoid dicarboxylic acid from the basidiomycete Schizophyllum commune with an inhibitory effect on squalene synthase (ICso 0.84 pM), a key enzyme of cholesterol biosynthesis. 

Plasma membranes from Schizophyllum commune protoplasts have been stabilized against fragmentation by coating the protoplasts with concanavalin Approximately half of the chitin synthase activity is associated with the plasma membranes in the form of a zymogen and could not be detached from the membranes. It is suggested that this inactive form represents the cytoplasmic transport form of the enzyme. The nature and location of proteases of Aspergillus nidulans and the interaction of one of these with the zymogen of chitin synthetase have been described. ... 

Figure 3 shows an example of enzyme analysis of the cellulolytic system of the fungus Schizophyllum commune which has been extensively... 

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