Scale-up Fermentation and Process Control of Bioisoprene

The scale-up fermentation of bioisoprene by engineered E. coli is summarized in Table 16.2. In these scale-up studies, suitable control of the fermentation process is essential for isoprene production due to the toxicity of the accumulating intermediate metabolites (GPP, FPP, IPP, DMAPP, MVPP). The accumulation of these intracellular metabolites can inhibit the growth of the hosts as well as its feedback inhibition on MVK. Therefore, a significant rate of isoprene biosynthesis cannot be reached or maintained for a long time under these conditions. In order 

BL21(DE3) GI1.2-Lower/pCXpTrcUpper/pTrcKudzu- yMVK(yeast) 6.4 2.8 [53, 54] 

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