Sanger, Frederick chain termination

The restriction fragments can be sequenced using a chain-terminator procedure developed by Frederick Sanger known as the dideoxy method. This method involves generating fragments whose length depends on the last base added to the fragment. Because of its simplicity, it has superceded alternative methods. 

In the chain termination or dideoxy method of DNA sequencing developed by Frederick Sanger, a primer-DNA template is divided into four separate reaction mixtures. To each is added the four dNTPs, one of which is labeled... 

Frederick Sanger, who invented the method, is among the few individuals to have won two Nobel Prizes. He received the Nobel Prize in Chemistry in 1958 for determination of the complete structure of the protein insulin and, in 1980, he received the Chemistry prize again for the discovery of the chain termination method of DNA sequencing (Chapter 14). 

Just after 1975, several methods for DNA sequencing were developed. Two of them, the chemical cleavage method of Allan Maxam and Walter Gilbert, and the chain terminator method of Frederick Sanger, are today used. The chemical cleavage method is explained below. 

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