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Maxam-Gilbert sequencing method

The pathway for strand cleavage using the Maxam-Gilbert sequencing method (1) with a thymidine residue and the cleavage method of the current invention are illustrated in Eqs 2 and 3, respectively. [Pg.339]

Modified Maxam-Gilbert Sequencing Method According to the Current Invention... [Pg.339]

What is the main difference between the Sanger dideoxy method and the Maxam-Gilbert sequencing method ... [Pg.418]

TABLE 10.4. Labeled Fragments Expected from the 32P-ACTGTAGC Cleavage Using the Maxam-Gilbert Sequencing Method... [Pg.204]

Determination of DNA Sequence Information. Almost all DNA sequence is determined by enzymatic methods (12) which exploit the properties of the enzyme DNA polymerase. Whereas a chemical method for DNA sequencing exists, its use has been supplanted for the most part in the initial determination of a sequence. Chemical or Maxam-Gilbert sequencing (13) is more often used for mapping functional sites on DNA fragments of known sequence. [Pg.233]

In principle, the Maxam-Gilbert method can provide the total sequence of a dsDNA molecule just by determining the purine positions on one strand and then the purines on the complementary strand. Complementary base-pairing rules then reveal the pyrimidines along each strand, T complementary to where A is, C complementary to where G occurs. (The analogous approach of locating the pyrimidines on each strand would also provide sufficient information to write the total sequence.)... [Pg.362]

The Maxam-Gilbert method doesn t require synthesis of a primer and it sometimes works well for sequences that are difficult to obtain with the Sanger -Coulson procedure. The two methods may both be used to provide additional certainty about a sequence. The Maxam-Gilbert method is very convenient for sequencing small oligonucleotides which often react poorly with the polymerase used for the chain termination method. The method usually requires that a restriction map be prepared. [Pg.264]

Sequence analysis of nucleotides by the Sanger or Maxam-Gilbert method... [Pg.420]

Chain extension with reverse transcriptase. Sequence by Maxam -Gilbert method (chapter 5)... [Pg.216]

Since its introduction in 1977 this has become the most widely used sequencing procedure for DNA. In the intervening period the method has been refined and developed to the point where it has become the method of choice in laboratories all over the world. Though not as fast as the primed synthesis methods, it is, up to the time of writing the most widely tried and tested procedure and the majority of DNA sequences reported in the literature to date have been determined by the Maxam-Gilbert procedure. [Pg.230]


See other pages where Maxam-Gilbert sequencing method is mentioned: [Pg.339]    [Pg.59]    [Pg.655]    [Pg.339]    [Pg.59]    [Pg.655]    [Pg.296]    [Pg.409]    [Pg.295]    [Pg.6]    [Pg.267]    [Pg.278]    [Pg.296]    [Pg.424]    [Pg.205]    [Pg.1181]    [Pg.1181]    [Pg.45]    [Pg.50]    [Pg.53]    [Pg.401]    [Pg.59]    [Pg.62]    [Pg.4]    [Pg.250]    [Pg.266]    [Pg.1188]    [Pg.122]    [Pg.434]    [Pg.122]    [Pg.434]    [Pg.260]    [Pg.1175]    [Pg.89]    [Pg.90]    [Pg.238]   
See also in sourсe #XX -- [ Pg.50 , Pg.53 ]

See also in sourсe #XX -- [ Pg.59 , Pg.62 ]

See also in sourсe #XX -- [ Pg.59 ]




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