Salmo irideus

Then 0.14 g of salmiridine sulfate dissolved in water is added, while shaking. Salmiridine is a protamine derived from the sperm of Salmo irideus Gibbons, or rainbow trout, Sal-miridine insulin (a protamine-insulin) containing zinc is promptly precipitated. Enough water is now added to make a total of one liter, and the whole is shaken again. After standing for about an hour, the precipitated salmiridine-insulin is found to have become crystalline. 

Georgiev, G.S. (1971). Carotenoids and vitamin A content in Salmo irideus eggs and their significance in the initial periods of the embryogenesis. Folia Balcanica 2(9), 11pp. 

Fluometuron is moderately toxic to fish and to animals consumed by fish. The Lc,p limit for rainbow trout /Salmo irideus) is more than 60 ppm, for water flea (Gommarus pulex) 60 ppm. Exposure of killifish (Orysias latipes) to 25 ppm of fluometuron for 25 hours caused a mortality of 6.7% to 50 ppm a mortality of 100% (Ciba, 1966 Brade, 1%8). 

Dawood, A. A., Karkalas, J., Roy, R. N., and Williams, C. S. (1988). The occurrence of nonvolatile amines in chilled-stored rainbow trout salmo irideus). Food Chem. Tl, 33-45. 

In a recent study Chiancone et al. [469] investigate the Cl binding to the two main hemoglobin components in trout blood, Hb-Trout I and Hb-Trout IV. These two hemoglobins from the species Salmo irideus are electrophoretically different and may be separated. The two components have strikingly different functional properties. A Root effect (the equivalent of the Bohr effect for human hemoglobin),... 

Salmo irideus (Trutta iridis) (Rainbowtrout) Iridine MP [DP (Lys)] Felix and Mager, 1937... 

Protamines of trout family Iridine Salmo irideus... 

Quite similar results have also been obtained recently with whole clupeine from Clupea harengus (Nukushina, 1964 Nukushina et al, 1964), whole salmine from Oncorhjnchus keta and iridine from Salmo irideus (Watanabe, 1969 Ando and Wata-NABE, 1969 Watanabe and Ando, to be published). Thus, a few components having similar but not identical structures are expected to be present in each of these unfractionated protamines, clupeine, salmine, and iridine. 

In an attempt to trace the origin of the heterogeneity, clupeine and iridine specimens were prepared under exactly the same mild conditions from completely mature testes of individual specimens of Clupea pallasii (Ando and Sawada, 1959, 1962) and Salmo irideus (Ando and Sawada, 1959, 1960), respectively. They used three Pacific herring caught at three different places off Hokkaido and three rainbow trout obtained... 

Fig. VII-8. Column chromatographic fractionation on preparative scale of protamines into all or some of their components using CM-Sephadex C-25 or Bio-Gel CM-2. A. Protamine ca. 25 mg of whole clupeine sulfate (from Clupea pallasii) in a small amount of water. Column 0.9 X 130 cm of Bio-Gel CM-2 (high capacity). Elution 0.05 M acetate buffer, pH 5.8, containing 1.5 M NaCl at room temperature at a flow rate of 2.4 ml/h. B. Protamine ca. 50 mg of whole salmine sulfate (from Oncorhynchus ketd) in a small amount of water. Column 0.9 X 150 cm of Bio-Gel CM-2 (high cap.). Elution 0.05 M acetate buffer, pH 5.8, containing 1.0 M and 1.5 M NaCl at room temperature at a flow rate of 10 ml/h. C Protamine ca. 600 mg of whole iridine sulfate (from Salmo irideus) in as small as possible amount of the eluting buffer containing 0.5 M NaCl. Column 3.0 x 146 cm of CM-Sephadex C-25. Elution 0.05 M acetate buffer, pH 5.8, containing 1.5 M NaCl at room temperature at a flow rate of 85 ml/b (Reproduced from Ando, T., and Watanabe, S., 1969)...

The biosynthetic development of protamine was first studied by analysis of amino-acid composition and terminal groups of proteins in the nuclei fraction from the testis of rainbowtrout (Salmo irideus) at different stages of spermatogenesis, as shown in Table X-2. These studies showed that in the immature stage DNA is bound to basic proteins of the histone type, which are gradually replaced as maturation proceeds by basic proteins of the protamine type, so that in the fully matured sperm heads DNA is bound only to protamine [Ando and Hashimoto, 1958 (1 2, 3) Felix 1958 Felix, I960]. 

Ando, T., Hashimoto, C. (2) Studies on protamines. V. Changes of the proteins in the cell nuclei of the testis during the formation of spermatozoa of the rainbow-trout (Salmo irideus). J. Biochem. (Tokyo) 45, 529—540 (1958). 

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