Saccharomyces cerevisiae isomerase from

Dobson MJ, Roberts NA, Tuite ME et al. (1983) Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae. Gene 24(1) 1-14 Doelle HW (1994) Microbial process development. World Scientific, Singapore, pp 241-276 Doha SC, Gaikar VG (2006) Aqueous to-phase partitioning of glucose isomerase from Actino-planes missouriensis in the presence of PEG-derivatives and its immobilization on chitosan beads. Separ Sci Technol 41(12) 2807-2823... 

It has been shown that an isomerase from fat-degrading plant tissue was entirely restricted to the peroxisomes and the isomerase activity could be attributed to a homodimer with a molecular mass of 50kDa. ° Interestingly, Erdmann and co-workers have recently demonstrated a peroxisome-associated isomerase activity in Saccharomyces cerevisiae cells grown on oleic acid as carbon source, however the properties of the corresponding protein have not been characterized. 

GDP-mannose pyrophosphorylase (GMP, EC 2.7.7.13). The recombinant bifimctional PMI/GMP (phosphomannose isomerase/GDP-D-mannose pyrophosphorylase) from Helicobacter pylori has been cloned and overexpressed in E. coli (21), The bifunctional enzyme PMI/GMP catalyzes both the first and third steps of GDP-D-mannose biosynthesis from D-fructose-6-phosphate. In addition, PMM of H. pylori was also cloned and overexpressed (21), The system was coupled with a truncated al,2-mannosyltransferase (ManT catalytic domain, Saccharomyces cerevisiae) to synthesize the mannosyloligosaccharides (not published). 

Madhavan A, Tamalampudi S, Ushida K, Kanai D, Katahira S, Srivastava A, Fukuda H, Bisaria VS, Kondo A. (2009b). Xylose isomerase from polycentric fungus Orpinomyces gene sequencing, cloning and expression in Saccharomyces cerevisiae for bioconversion of xylose to ethanol. Appl Microbiol Biotechnol, 82, 1067-1078. 

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