Rubredoxin temperature dependence

Figure 2. Temperature dependence of the MCD intensity of Ni(II)-substituted rubredoxin from Desulfovibrio gigas at 664 nm. Taken from Ref. 16.

From the biological area, iron-sulfur clusters in biomolecules such as rubredoxin mononuclear Fe-S clusters (Rao et at., 1972), plant-type ferredoxin 2Fe-2S clusters (Johnson, 1975) and bacterial-type ferredoxin 4Fe-2S clusters (Thompson et at., 1974) are readily distinguished from one another by their Mossbauer spectra. The temperature dependence of relaxation effects can provide information about the types of internuclear interaction and can even lead to estimates of the distance between paramagnetic sites, for example, the two 4Fe-4S clusters in ferredoxin in Peptococcus aerogenes (Adman etal., 1973). 

Rg. 2. Temperature dependence of the activity of P. furiosus NROR. Activities were measured at the indicated temperature using NADPH as the electron donor and either P. furiosus rubredoxin (o) or benzyl viologen ( ) as the electron acceptor. For the rubredoxin assay, the 2 ml reaction mixture contained 100 mAf EPPS pH 8.0, rubredoxin (9.5 ]t,M) and NADPH (0.3 mAf). For the benzyl viologen assay, the 2 ml mixture contained 50 mA CAPS buffer, pH 10.2, benzyl viologen (1 mAf) and NADH (0.3 mAf). 

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