Rubidium efflux

Sorota, S., Zhang, X.S., Margulis, M., Tucker, K., and Priestley, T., Characterization of a hERG screen using the Ion Works HT comparison to a hERG rubidium efflux screen, Assay Drug Dev. Technol., 3, 45-57, 2005. 

Marks MJ, Smith KW, Colhns AC (1998) Differential agonist inhibition identifies multiple epiba-tidine binding sites in mouse brain. J Pharmacol Exp Ther 285 377-386 Marks MJ, Whiteaker P, Calcaterra J, Stitzel JA, Bullock AE, Grady SR, Picciotto MR, Changeux JP, CoUins AC (1999) Two pharmacologically distinct components of nicotinic receptor-mediated rubidium efflux in mouse brain require the beta2 subunit. J Pharmacol Exp Ther 289 1090-1103... 

Chaudhary, KW., O Neal, f.M., Mo, Z.L., Fermini, B., Gallavan, R.H. and Bahinski, A. (2006) Evaluation of the rubidium efflux assay for preclinical identification of hERG blockade. ASSAY and Drug Development Technologies, 4, 73—82. 

Note 2 The authors of the reference protocol additionally compared the IC50 values obtained by the rubidium efflux assay, electrophysiology assay, and dofetilide displacement assay for astemizole, E-4031, cisapride, terfenadine, risperidone, quinidine, and sotalol, and the obtained values are demonstrated in Table 1. 

Scott CW, Wilkins DE, Trivedi S, Crankshaw DJ (2003) A medium-throughput functional assay of KCNQ2 potassium channels using rubidium efflux and atomic absorption spectrometry. Anal Biochem 319(2) 251-257... 

Terstappen, G.C. Functional analysis of native and recombinant ion channels using a high-capacity nonra-dioactive rubidium efflux assay. Anal. Biochem. 1999, 272, 149-155. 

Burgen, A. S. V., and Spero, L. (1968). The action of acetylcholine and other drugs on the efflux of potassium and rubidium from smooth muscle of the guinea-pig intestine. Br. J. Pharmacol., 34 99—115. 

Interestingly, the two halves bind with much less affinity but cause an efflux of rubidium, suggesting that both act as agonists. Furthermore, acting in a similar fashion to lobeline, the effects of the compounds could not be reversed by mecamylamine or other nicotinic antagonists. These compounds also exhibited a similar pharmacological profile to lobeline in... 

Kresca, L., Cotlier, E. Invest. Ophthalmol 13 (4), 310 (1974). Valinomycin-stimulated 86 -rubidium transport and efflux from lens... 

Lukas, R., and Cullen, M. (1988). An Isotopic Rubidium Ion Efflux Assay for the Functional Characterization of Nicotinic Acetylcholine Receptors on Clonal Cell Lines, . 4na/, Biochem. 175 212—218. 

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