Rows of Spots

The District of Columbia has long been concerned about rows of spots in numerous locations on the 1918 aerial photograph. Recently, a RAB member discovered test reports that indicated that dogs were chained to stakes in groups of 20, four rows of five. The District of Columbia believes for a variety of reasons that these tests took place at POI 7, because of the 

Originally, the District of Columbia thought that the spots might represent holes where poles were placed. Several test reports indicate that shells were statically bred while attached to the ends of 10-ft poles, probably to simulate shells striking trees in a forest. This may still be a plausible explanation for some spots in other locations. A newly discovered document in the archives offers another explanation, especially for the spots that are near known shell storage areas these could be pre-dug holes to bury leaking chemical shells. 

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A sketch of the perforated lamellar morphology is depicted in Fig. 2. Depending on the point of view, the 2D projection exhibits different patterns perpendicular to the layers of the perforated lamellar structure the projection appears like a hexagonal honeycomb mesh (Fig. 2a). By contrast, the parallel view (Fig. 2b) leads to rows of spots. 

Variations of the White cell are also in use. For example, Horn and Pimentel (1971) added a corner mirror assembly to redirect the beam that would normally exit the cell back into it. This doubles the number of passes, giving four rows of spots on the field mirror. The image pattern for such a design is shown in Fig. 11.1c (Hanst and Hanst, 1994). 

If row lines are not shown, then the crystals are triclinic. The inclination of c to the vertical may be shown by a row of spots in line with the origin but the orientation of c with regard to the zero-level net must be found by trial. Distances equal to the f values for 00 planes are marked off along a line on a strip of paper pivoted at the origin of the zero-level net, and this line is swung round until the distances measured, as in Fig. 94, correspond with the values on the rotation diagram. This process is not so difficult or lengthy as it may seem. 

Keeping the center of the traced pattern (reproducing the spots and axes of the film) carefully to the center of the chart, any row of spots can be made to coincide with one of the hyperbolas extending horizontally across the chart by turning it through an angle a, where a is the azimuthal angle. 0 is determined directly from the chart (Fig. 8). 

On the 1918 aerial photograph, this area has a double row of troughs transecting the field at its western portion. A cross set of double trenches crosses the field at its northern portion. South of and along this double set of trenches are three rows of spots. These are believed to be either post-holes where posts were set in the ground for tests of shells fastened to their tops or stakes to which dogs were fastened. 

Fig. 16. Electron diffraction pattern showing rows of spots with a bent configuration.

Fig. 17. A micrograph of the crystal of which the electron diffraction pattern is shown in fig. 16. The bend of the crystal corresponds to that of the rows of spots.

The array above has 15 rows of spots containing synthetic capture RNA covalently attached to a glass slide. Three spots in each row contain identical capture RNA designed to bind to a short section of one strain of viral RNA. The spot at the left in each row is a control that will become fluorescent in every test and serves as an internal standard. Viral RNA extracted from patients is amplified (reproduced into many copies) and digested (cleaved into fragments). Capture RNA on the slide binds selected viral RNA fragments. Another synthetic RNA with a fluorescent tag is designed to bind to a different section of viral RNA. After allowii d ested viral RNA to bind to capture RNA and to fluorescent RNA, excess fluorescent RNA is washed away. Fluorescence intensity in each spot is related to the amount of viral RNA bound at that spot. 

Fig. 10. Contrast enhanced pictures of the patterns, (a) Triple front pattern (b) Multiple front pattern (c) Single row of clear spots (d) Three rows of spots.

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