Robustness chemokines

Mantovani, A. (1999). The chemokine system Redundancy for robust outputs. Immunol. Today 20, 254-257. 

Mantovani A. The chemokine system redundancy for robust outputs. Immunol Today 1999 20 254-257. 

There are many approaches to producing functional recombinant chemokines from E. coli in addition to the ones referenced or presented here. The goal of this work is to describe a robust protocol we employ for many different chemokines, which draws from a variety of previously described approaches. We illustrate the impact of disulfide shuffling cocktails on the... 

In conclusion, the supply of pure, natively folded chemokine proteins is vital for a variety of basic, translation and cHnical research programs. Our structure-function studies and drug development efforts have motivated the development of the robust protocol for recombinant chemokine expression, refolding, purification, and vahdation presented here. Finally, many additional factors must be considered when manufacturing a product under cGMP guidehnes that may not be obvious to personnel in research settings. 

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