RNA, cleavage

DNA and RNA Cleavage by Metal Complexes Genevieve Pratviel, Jean Bernadou, and Bernard Meunier... 

Fig. 10A pL (L=H or D) profiles of hairpin ribozyme of the native sequence of and 2,6-diaminopurine at position 8 [119]. The native sequence in H2O and in D2O are represented hy filled diamonds and filled squares, respectively. 2,6-diaminopurine at position 8 in H2O and in D2O are represented by open circles and open triangles, respectively. B Proposed catalytic mechanism. RNA cleavage catalyzed by the keto-enol tautomeriza-tion of Gg is achieved by a abstraction of a proton from the N1 to the 5 -0 leaving group [119]... 

Meister G, Landthaler M, Patkaniowska A et al (2004) Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell 15 185-197... 

O. (2004) Synthetic metallonucleases for RNA cleavage. Curr. Opin. Chem. Biol., 8, 192. (e) Molenveld, P., Engbersen, J.F.J. and Reinhoudt, D.N. (2000) Dinuclear metallophosphodiesterase models application of calix[4]arenes as molecular scaffold. Chem. Soc. Rev., 29, 75. (f) Kimura, E. (2000) Dimetallic hydrolases and their models. Curr. Opin. Chem. Biol., 4,207. (g) Williams, N.H., Takasaki, B. and Chin, J. (1999) Structure and nuclease activity of simple dinuclear metal complexes quantitative dissection of the role of metal ions. Acc. Chem. Res., 32, 485. (h) Hegg, E.L. and Burstyn, J.N. (1998) Toward the development of metal-based synthetic nucleases and peptidases a rationale and progress report in applying the principles of coordination chemistry. Coord. Chem. Rev., 173, 133. 

RNA catalysis is not only concerned with RNA cleavage non-natural ribozymes that show ligase activity (Bartel and Szostak, 1993) were obtained and many (so far not yet successful) efforts have been undertaken to prepare a ribozyme with RNA replicase activity. RNA catalysis does not only operate on RNA, nor do nucleic acid catalysts require the ribose backbone. Ribozymes were trained by evolutionary techniques to process DNA rather than their natural RNA substrate (Beaudry and Joyce, 1992), and catalytically active DNA molecules were evolved as well (Breaker and Joyce, 1994 Cuenoud and Szostak, 1995). Systematic studies revealed many other examples of RNA catalysis on non-nucleic acid substrates (see... 

Deoxyribozymes available for different RNA cleavage-site sequences... 

---