Rigorous Optimization for Isocratic Runs

After developing a first gradient method, the optimization of an isocratic method should be considered for process-scale separations. Gradient runs help to find 

Easier equipment can be used (only one pump, lower number of eluent tanks). 

No re-equilibration time necessary (touching band-methods as well as closed- 

Easier eluent recycling due to constant eluent composition. 

In the following paragraph the development of a separation method is explained in detail by the separation of a pharmaceutical sample consisting of three intermediates. 

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