Rhodococcus rhodochrous ATCC

In the hydroxylation of n-propane to n-propanol by Nocardia paraffinicum (Rhodococcus rhodochrous) ATCC 21198, the ratio of hydrocarbon to oxygen consumed was 2 1 and this suggests that the reaction of two molecules of propane and one molecule of dioxygen... 

The preparation of malonic acid monoesters has been demonstrated using the microbial nitrilase activity of Corynebacterium nitrilophilus ATCC 21 419, Gordona terrae MA-1, or Rhodococcus rhodochrous ATCC 33 025 to hydrolyze methyl cyanoacetate, ethyl cyanoace-tate, M-propyl cyanoacetate, isopropyl cyanoacetate, M-butyl cyanoacetate, tertbutyl cyanoacetate, 2-ethylhexyl cyanoacetate, allyl cyanoacetate, and benzyl cyanoacetate [96]. By maintaining the concentration of nitrile in a reaction mixture at <5 wt%, significant inactivation of the nitrilase activity was avoided for example, a total of 25 g of M-propyl cyanoacetate was added in sequential 5g portions to a lOOmL suspension of Rhodococcus rhodochrous ATCC 33 025 cells (OD630 = 5.6) in 50 mM phosphate buffer (pH 7.0) over 30h at 25 °C to produce mono-M-propyl malonate in 100% yield (Figure 8.17). 

Figure 8.17 Production of mono-n-propyl malonate from M-propyl cyanoacetate using the microbial nitrilase activity of Rhodococcus rhodochrous ATCC 33025...

Kilbane n, J. J., Enzyme from Rhodococcus rhodochrous ATCC 53968, Bacillus sphaericus ATCC 53969 or a mutant thereof for cleavage of organic C-S bonds. Patent No. US5516677. 1996, May 14. 

The microbial reduction of 4-benzyloxy-3-methanesulfonylamino-2 -bromoace-tophenone (6) to the corresponding (A)-alcohol (7) was demonstrated by S. paucimobilis SC 16113 (Fig. 6). Among cultures evaluated, Hansenula anamola SC 13833, H. anamola SC 16142, Rhodococcus rhodochrous ATCC 14347, and S. paucimobilis SC 16113, gave desired alcohol (7) in >96% e.e. and >15% reaction yield. S. paucimobilis SC 16113, in the initial screening, catalyzed the efficient conversion of ketone (6) to the desired chiral alcohol (7) in 58% reaction yield and >99.5% e.e. 

Kinfe, H.H., Chhiba, V., Erederick, J., et al. 2009. Enantioselective hydrolysis of 3-hydroxy nitriles using the whole cells biocatalyst Rhodococcus rhodochrous ATCC BAA-870. Journal of Molecular Catalysis B Enzyme, 59 231-6. 

Figure 11.3 Biotransformations of p-hydroxy nitriles and analogs by nitrile hydratase and amidase in whole cells of Rhodococcus rhodochrous ATCC BAA-870 [15]. To determine the enantiomeric excess, both p-hydroxy amides and p-hydroxy acids were separately converted into the corresponding methyl ester using Fischer esterification.

Ghhiba, V.P., Bode, M.L., Mathiba, K., Kwezi, W., and Brady, D. (2012) Enantioselective biocatalytic hydrolysis of P-aminonitriles to P-amino-amides using Rhodococcus rhodochrous ATCC BAA-870. J. Mol. Catal. B Enzym., 76, 68-74. 

Figure 10 Synthesis of (S)-2-arylpropionic acids by an (S)-specific nitrilase from Rhodococcus rhodochrous ATCC 21197.

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