Lipase rhizopus arrhizus

Figu re 1.4 Normalized reaction rate as function of water activity for the esterification of dodecanol with decanoic acid catalyzed by Rhizopus arrhizus lipase at three different concentrations 20mM (O), 200mM ( ) and 800mM ( ) of each substrate. Data obtained from Ref. [26]. 

Rhizopus arrhizus lipase (enantioselective) Rhizopus nigricans mold (enantioselective) Rhizopus niveus lipase (enantioselective)... 

When 80 20 blends of butter fat and canola oil were used, chemical interesterification increased the solid fat content above 10°C while enzymatic interesterification by Rhizopus arrhizus lipase reduced solid fat content over the range 5 40 C (Rousseau and Marangoni, 1999). 

Rousseau, D., Marangoni, A.G. 1998a. Tailoring attributes of butter fat/canola oil blends via Rhizopus arrhizus lipase-catalyzed interesterification. 1. Compositional modifications. J. Agric. Food Chem. 46, 2368—2374. 

Verhaeghe, D., Ferijn, H., Huyghebaert, A., de Moor, H. 1990. Controlled lipolysis of milk fat with Rhizopus arrhizus lipase. Milchwissenschaft 45, 275-278. 

Tokiwa, Y., Suzuki, T. Takeda, K. (1986). Hydrolysis of polyesters by Rhizopus arrhizus lipase. Agricultural and Biological Chemistry, 50, 1323-5. 

Recently, a new approach using enzymes to modify material (or nanomaterial) surfaces has been developed. This approach utilizes enzymatic hydrolysis or degradation to mildly etch the material surfaces and thus CTeate desirable surface structures or properties [45]. Many polymers, including polyvinyl chloride (PVC), polyaayloni-trile, polyethylene terephthalate, and polyamides, have been modified by this method. In a recent study, PVC tubes were soaked in Rhizopus arrhizus lipase to create nanometer surface features that exhibited antibacterial properties [60]. Enzymatic modification demonstrates great potential and promise for biomedical and nanomedicine applications due to its biocompatible, environmentally friendly, and simple process. 

Rousseau, D, AG Marangoni. (1998). TaHoring the textural attributes of butterfat-canola oil blends via Rhizopus arrhizus lipase-catalyzed interesterification 11. Modification of physical properties. J Agric Food Chem 46 2375-2381. 

Fig. 13. (A) Action of cobra venom phospholipase Aj on mixed micelles consisting of 15 mM dipalmitoyl-PC (PC) and 120 mA/ Triton X-100. The enzyme concentration was 12.5 ng ml. The solution contained 10 mMCaCl2, 20% DjO, and SO mA/Tris-HO buffer, pH 6.0. (B) Action of Rhizopus arrhizus lipase (375 /ig ml ) under identical conditions. Spectra were obtained using the spectrometer described in Fig. 2. From Pliickthun and Dennis (1982a). Copyright 1982 American Chemical Society. Note that the original figure has been altered to now show positive chemical shifts in the direction of decreasing field strength.

Methyl Rhizopus arrhizus lipase from Boehringer Manheim 120 22 11 97 — 13 78 77... 

H- n-Butyl Rhizopus arrhizus lipase (Fluka)/buffer-saturated isooctane 513 37 3.4 99 2.1 >100 99... 

Brown, E., Yada, R., and Marangoni, A. (1993) The dependence of the lipolytic activity of Rhizopus arrhizus lipase on surfactant concentration in Aerosol-OT/isooctane reverse micelles and... 

---