Rezex ROA Organic Acid

We experimented firstly with a Phenomenex Rezex ROA-Organic Acid 300 X 7.8 mm column with a Rezex Organic Acid 50 x 7.8 mm guard column the mobile phase was 0.013 N (0.0065 M) H2SO4. UV detection was at 215 nm, the flowrate 0.6 ml min, column temperature 35°C and injection volume 20 pi. The internal standard was 2-ethylbutyric acid. The lactic acid peak was preceded by a partially overlapping, probably succinic acid peak the... 

Fig. 8.5. HPLC chromatogram of a first-out silage juice using a Rezex ROA-organic acid column. Identity a, laotio aoid b, formic acid c, acetic acid d, propionic acid e, n-butyric acid f, 2-ethylbutyric acid (internal standard).

Rezex ROA Organic Acid Phenomenex 300 x8 8 6.9 Sulfonate groups High-performance separator for weak organic acids alone or in combination with carbohydrates and alcohols... 

Figure 5.7 Comparison of resolution and peak 0.5 mmol/L H2SO4 flow rate 0.5 mL/min shapes for selected organic acids between detection UV (210nm) peaks oxalic acid (1), Rezex ROA Organic Acids and Aminex HPX- citric acid (2), tartaric acid (3), succinic acid (4),...

Other widely used ion-exclusion columns for the analysis of organic acids in combination with carbohydrates and alcohols are Aminex HPX-87 H (Bio-Rad, Hercules, CA, USA) and Rezex ROA (Phenomenex, Torrance, CA, USA), which are typically operated at elevated temperatures for optimal resolution. Both column substrates are totally sidfonated poly(styrene-co-divinyl-benzene)-based resins with a degree of cross-linking of 8% that slightly differ in particle size. Figure 5.7 illustrates the better resolution and peak shapes with less fronting effects for organic acid analysis on the Rezex ROA colunrn in comparison to the Aminex HPX-87H, while the overall analysis times are comparable. 

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See also in sourсe #XX -- [ , ]

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