Reversed phase media aqueous buffer

After renaturation, the majority of the recombinant chemokines are easily quantified by UV spectroscopy, although there are examples of chemokines such as NAP-2, which do not possess an aromatic amino acid that serve as chromophores. In this case, quantification is achieved by comparison of the peak height on reverse phase HPLC analysis to that of a known concentration of another chemokine. They can then be lyophilized after a change of buffer into a trifluoroacetic acid or acetic acid solution, which facilitates their storage as lyophilized powders. It is important that they are redissolved in water, before dilution into buffer or medium. Their handling is easy and rapid, as they are instantly soluble at concentrations as high as 1 mM if necessary, in aqueous solutions. 

Medium polar packings Between polar and non-polar Silicas with bonded propylcyano groups, silicas with bonded diol groups, silicas with bonded amino groups Buffered aqueous /organic mobile phases (reversed phase conditions) moderately polar organic mobile phases... 

Reversed phase (Cu, Cg, C4, C2, phenyl, diphenyl, cyclohexyl, cyano-propyl) for nonpolar analytes (alkyl, aromatic) in polar solutions (water, aqueous buffers), with high to medium polarity eluents (methanol, acetonitrile, water)... 

---