Reversed-phase HPLC chromatogram

Fig. 2.3.7. Overlay of reversed-phase HPLC chromatograms of branched and linear octylphenol, and NP[EO]9. Column 125x4 mm2 Lichrospher RP-18 (5 pm), isocratic elution 1.0 mL min-1, mobile-phase 8 2 (v/v) methanol/water. 

Fig. 2.8. Reversed-phase HPLC chromatograms (430 nm) of colour pigments of paprika powder after 56 days of storage time, a = control, b = 5 per cent GLT, c = 5 per cent HP-/3-CD. Peak indentification 1 = capsanthin 2 = zeaxanthin 3 = /3-carotene. Reprinted with permission from T. Cserhati el al. [32].

Fig. 2.161. Superimposed reverse-phase HPLC chromatograms of mixtures containing (lower) bilirubin XHIa, IXa and Ilia and (upper) diphenyl bilirubins XHIa (2), IXa (1) and Ilia (3). The mobile phase was 0.1 M di- -octylamine acetate in 8 per cent water/methanol at a flow rate of 0.75 ml/min. Reprinted with permission from J. O. Brower et al. [333],...

Fig. 3.10 Reverse-phase HPLC chromatogram of PA phloroglucinol cleavage products from the polymer-rich fraction of GSE...

Fig. 7. Reversed-phase HPLC chromatogram of an aqueous solution of iotrolan. a Low resolution [ODS-Hypersil, 5 pm, 250 x 4.6 mm water - methanol, 500 80 (v/v)]. b Low resolution (Superspher 100, 250x4 mm, water pH 3.4 (NH4-Formiate, 0.02 M), Na-heptane sulfonate (2 g L ), 1.5 mL min 40°C) Numbers 1-5 indicate the peak numbers, c High resolution (Superspher 100, 250x4 mm, water/methanol, 900 100, 0,4 mL min 40°C) Numbers 6-17 indicate the peak numbers...

Figure 5.4 Reversed-phase HPLC chromatogram of a sample of the complex alkaloid cryp-tospirolepine (11) that had been stored in de-DMSO in a sealed NMR tube for a period of 10 years. None of the starting alkaloid remains there are a total of 26 components observed in the chromatogram, the two major peaks at 16.591 and 18.123 min retention times constituting >45% of the degraded sample.

Figure 6. Reverse-phase HPLC chromatograms from XAD-2/ethyl ether extracts of (a) chlorinated phenylalanine and (b) chlorinated tyrosine. An eluent of 55% methanol in water was used in (a), and an eluent of 35% methanol in water was used in (b).

Fig. 2 Reversed-phase HPLC chromatogram of the dansyl derivatives of amines from a sample of spoiled table olives. Peak identities 1, putrescine 2, cadaverine 3, 1,7-diaminoheptane (IS) 4, tyramine. (From Ref. 19.)...

Figure 1. Reversed-phase HPLC chromatograms of human relaxin side fraction before and after reduction with dithiothreitol. The chromatography was performed on a Vydac C4 column using TFA-containing mobile phases, and eluted with an acetonitrile linear gradient from 18 to 50%.

Figure 1.4. Reversed-phase HPLC chromatogram of milk fat triacylglycerols (from Robinson and MacGibbon, 1998).

Figure 9.37 Isocratic reversed-phase HPLC chromatogram showing separation and detection of dihydroquinozolinium (DHQ) and o-aminobenzaldehyde (OAB). The column was LiChrosorb Qg, and 4.6 mm X 250 mm, and the solvent system was methanol-water (1 2), pumped at 1.5 mL/mm. Detection was at 254 nm with an Altex 310 chromatograph. (From O Donnell et al., 1978.)...

Figure 10.5 Effects of Na2S04 on fate of ATP. A 30 fig sample of the extract was added to reaction mixtures containing, in a total volume of 200 fiL, 100 fiM ATP, 0.4 mM Mn2+, 50 mM Tris-HCl (pH 7.4), and 0 to 200 mM sodium sulfate. The reaction was incubated at 31°C for 2 hours and terminated by heating to 155°C. Samples were analyzed by reversed-phase HPLC. Chromatograms represent an analysis of each reaction mixture 2 hours after the start of the reaction at four sulfate concentrations. Inset Initial rate of AMP and ADP formation from ATP. (From Rossomando and Jahngen, 1983.)... 

Figure 1. Reversed-phase HPLC chromatogram of a bench-scale refold mixture. The refold procedure was as described for the gram amount production lots for preliminary animal testing. Active rSLPI is at 13 min. retention, while the fully unfolded protein is at 18-19 min. and the partial or incorrectly folded species are found in between.

Figure 10. Reverse phase HPLC chromatograms of (a) a standard avidin solution and (b) Fraction B from ion-exchanged partially purified egg whites (described in experimental section). Continued on next page.

Figure 10. Continued. Reverse phase HPLC chromatograms of (c) supernatant of Fraction B following affinity precipitation and (d) reconstituted avidin from egg whites following affinity precipitation.

Fig.l. Reversed-phase HPLC chromatograms of methanolic sludge amended soil extracts of samples collected a few hours (A), 22 days (B), and 322 days (C), respectively, after the sludge apphcation. Cll, C12, C13, and C14 LAS homologs IS internal standard, 3-C 15-LAS. 

Figure 1.5. A reversed-phase HPLC chromatogram of three organic components eluting in the order of polar first and nonpolar last. The basic pyridine peak is tailing due to a secondary interaction of the nitrogen lone-pair with residual silanol groups of the silica based bonded phase. Figure reprinted with permission from reference 8, Chapter 2.

Figure 1. Reversed-phase HPLC chromatogram with monitoring at 520 nm of wine prior to wine tannin isolation. Inset shows detail of the baseline. (Reproducedfrom reference 16, with permission from the Australian Society of Viticulture and Oenology)...

Approximately 11% by weight of the parent material could be extracted using carbon dioxide at 60°C up to a pressure of 648 bar. A summary of a carbon dioxide extraction of GAP L-11391 is given in table 9.23. Reverse phase HPLC chromatograms which demonstrate the removal of the low molecular weight species from the parent material are given in figure 9.32. 

Fig. 1. Reversed phase HPLC chromatogram of an endoproteinase Lys C digest of PrP 27-30, showing absorbance at 214 and 280 nm versus time (min). The gradient line shows acetonitrile content. The peptide compositions shown for each peak were derived from the experiments described in Section III. Peptides were identified containing all residues between 74 and 231, with the exception of an anticipated tetrapeptide, residues 107-110. (Reproduced with permission from Stahl, N. et al. in Prusiner, S.B., Collinge, J., Powell, J., and Anderton, B. [1992], Prion Diseases of Humans and Animals, pp. 361-379. Copyright Elsevier Science).

Size exclusion and reverse phase HPLC chromatograms for some of the samples are compared in Figures 3 and 4. It is noted that samples H and G have a single major component i.e., the TGMDA monomer. Preparative LC concentrates the minor components and leaves only about of the TGMDA monomer in sample E. 

Figure 3. The reverse phase HPLC chromatograms of the pMMA, polystyrene, p(Sty-b-MMA) mixture with ELS and UV detection.

Figure 5. The reverse phase HPLC chromatograms of the mixture of pMMA, pBA, p(MMA/BA) and p(BA-b-MMA) with ELS and UV detections. The SUPELCOSIL Octadecyl column (25cm x 4.6mm ID) was used with acetonitrile/THF solvent gradient (flow rate Iml/min).

Fig. 1. Reversed phase HPLC chromatograms of pigment extracts of A. Rhodocyclus qelatinosus 151 B. Rhodomicrobium vannielii RB4...

In aqueous solutions, peptide aldehydes exist as an equilibrium state between the aldehyde (A) and hydrated forms (B). In the example presented here (Figure 14), leupeptin, the C-terminal arginal, can also cyclize to form the aminol (C). The same equilibrium states are seen in the reverse-phase HPLC chromatogram (three broad peaks). The electrospray mass spectrum shows only the [M + H]+ (A and C) and [M -I- H + 18 (HzO)] (B) peaks. 

Figure 2. A) Reverse-phase HPLC chromatogram of genistein (Peak B) reacted with HOBr with daidzein as an internal standard (Peak A). Two novel peaks are shown (C and D). B) 7%e UV-visible absorbance spectra of genistein (dotted line), peak C (dashed line), and peak D (bold line).

FIGURE 22.6 Reversed-phase HPLC chromatogram of 3 after purification on ion-exchange resin (for details see the General Methods section). 

Figure21.23 Reverse phase HPLC chromatograms of quinacridone pigments.

Figure 21.25 Reverse phase HPLC chromatogram of impurities in PR254.

Figure 1. Reversed phase HPLC chromatogram ofAlpinia officinarum 30 1 chloroform/methanol fraction at 205 nm using a Discovery CIS reversed phase HPLC column (250 mm x4.6 mm, 5 pm).

Fig 2. A, A typical reversed-phase HPLC chromatogram of a crude oligoribonucleotide (AUGAUCAUACA) prepared using conventionally protected ribophosphoramidites (Section 2,). B. The chromatogram of the same oligomer after purification by the sequential use of PAGE and Sephadex G25-F (Section 3.4,). HPLC conditions Section 3 4 6... 

---