Reversed-phase flow chart

Figure 4.27 Flow chart for coluwi selection based on sample type (m - molecular weight). PLC precipitation-liquid chromatography SEC = size-exclusion chromatography lEC - ion-exchange chromatography HIC hydrophobic interaction chromatography LSC liquid-solid chromatography RPC - reversed-phase liquid chromatography BPC (polar) bonded-phase chromatography and IPC - ion-pair chromatography.

FIGURE I 0 Impurity/degradant isolation and identification process flow chart (continued). RP, reverse phase NP, normal phase. 

Figure 2. Flow-chart for retention prediction in reversed-phase LC. Reproduced with permission from Ref. 10. Copyright 1984, Elsevier.

TIC chromatogram (bottom chart) and contour plot for the LC-ESI-MS analysis of Triton X-100, by using gradient reversed-phase chromatography [2 mm i.d. octadecylsrUca column, 20/80 to 50/50 acetonitrile/10 mM ammonium acetate in 20 min, 200 /iL/min flow rate, 3 1 effluent split, nebulizer-assisted electrospray]. See Figure 4.9 for the oligomer series (I-IV) separated. (Courtesy of PE Sciex, Foster City, CA)... 

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