Retaining glycosidases enzyme kinetics

Scheme 5.2 Minimal kinetic scheme for a retaining glycosidase. The double displacement reaction involves two enzyme-substrate complexes, the second of which is commonly (but not always) a glycosylated enzyme.

Figure 5.38 Burst kinetics showing liberation of aglycone during the establishment of a steady state during hydrolysis of a snbstrate by a retaining glycosidase, when the hydrolysis of the glycosyl-enzyme is rate determining. The glycone was fluorinated, so the experiment was performed in a conventional spectrometer (note time-scale). Note the burst of 0.03 absorbance units.

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