Restricted access media column

E. A. Hogendoorn, E. Dijkman, B. Baumann, C. Hidalgo, J. V. Sancho and E. Hernandez, Strategies in using analytical restricted access media columns for the removal of humic acid interferences in the trace analysis of acidic herbicides in water... 

Cass et al. [71] described a direct injection HPLC method, with column-switching, for the determination of omeprazole enantiomers in human plasma. A restricted access media of bovine serum albumin octyl column has been used in the first dimension for separation of the analyte from the biological matrix. The omeprazole enantiomers were eluted from the restricted access media column onto an amylose tris (3,5-dimethylphenylcarbamate) chiral column by the use of a columnswitching valve and the enantioseparation was performed using acetonitrile-water (60 40) as eluent. The analytes were detected by their UV absorbance at 302 nm. The validated method was applied to the analysis of the plasma samples obtained from 10 Brazilian volunteers who received a 40-mg oral dose of racemic omeprazole and was able to quantify the enantiomers of omeprazole in the clinical samples analyzed. 

EA Hogendoom, P van Zoonen, A Polettini, GM Bouland, M Montagna. The potential of restricted access media columns as applied in coupled-column LC/LC-TSP/MS/MS for the high-speed determination of target compounds in serum. Anal Chem 70 1362—1368, 1998. 

De Paula, F.C.C.R. De Pietro, A.C. Cass, Q.B. Simultaneous quantification of sulfamethoxazole and tri-methoprinin in whole egg samples by column-switching high-performance bquid chromatography using restricted access media column for on-bne sample clean-up. J. Chromatogr. A, 2008,1189, 221-226. 

Hogendoom, E. A., Huls, R., Dijkman, E., and Hoogerbmgge, R., Microwave assisted solvent extraction and coupled-column reversed-phase liquid chromatography with UV detection. Use of an anal)4ical restricted-access-medium column for the efficient multi-residue analysis of acidic pesticides in soils, J. Chromatogr. A, 938, 23-33, 2001. 

Oliveira RV, Cass QB, Evaluation of liquid chromatographic behavior of cephalosporin antibiotics using restricted access medium columns for on-line sample clean-up of bovine milk, J. Agric. Food Chem. 2006 54 1180-1187. 

---