Response Surface Methodology production compositions

Mu, Chen, Li, Zhang, and Jiang (2009) also smdied the effect of medium composition on the production of PLA by lactic acid bacteria. Attempting to optimize a medium for the commercial production of PLA, the authors used a response surface methodology and the results showed that addition of phenylpyruvic acid (PPA) increased the yield of PLA. The authors suggested that because the transamination step is a bottleneck in the synthesis of PLA, the addition of PPA allowed for a shortcut that bypassed the transamination of phenylalanine to PPA, which is normally the first step when phenylalanine is used as the source for PLA. 

Zhang, C.H., Ma, Y.J., Yang, F.X., Liu, W, Zhang, Y.D., 2009. Optimization of medium composition for but)fric acid production by Clostridium thermobutyricum using response surface methodology. Bioresource Technology 100 (18), 4284-4288. 

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