Resonance-excitation energy transfer

L. Davenport, R. E. Dale, R. H. Bisby, and R. B. Cundall, Transverse location of the fluorescent probe l,6-diphenyl-l,3,5-hexatriene in model lipid bilayer membrane systems by resonance excitation energy transfer, Biochemistry 24, 4097-4108 (1985). 

The electronic excitation discussed up to this point involves translational to electronic energy transfer. Of great practical importance is near-resonant electronic excitation-energy transfer, which leads to ion lasers in the He-Kr and Ne-Xe systems.294 The reactions involved are (He and Ne ... 

Details on the mechanisms and theories of excitation energy transfer via dipole-dipole interaction (FRET Forster resonance energy transfer) and via exchange interaction (Dexter s mechanism) can be found in B. Valeur, Molecular Fluorescence. Principles and Applications, Wiley-VCH, Weinheim, 2002, chap. 4 and 9. 

This energy function contains an integral term and a term determined by the nucleus configuration. We introduce the resonance integral for excitation energy transfer, Jab, in Eq. (1.150), where the subscripts 0 and 1 denote the ground and excited states, respectively. The quantity Jab is approximated by the interaction energy between the transition dipole moments, Pa and Pb, of the two molecules a and b, respectively, in the case for which the distance between the two molecules, Rab, is not too short. 

Electronic excitation energy transfer processes can be effective only very close to a resonance (0.1 eV or less), which limits them to some specific collision partners. The He-Ne gas laser provides examples ... 

Nonradiative relaxation from states can be effected via fluorescence resonance energy transfer. The rate of energy transfer in donor-acceptor pairs is directly related to their distance, which can of course be affected by analyte complexation. Excitation energy transfer leads to quenching of the donor, but also to enhancement of the acceptor fluorescence therefore, the ratio of fluorescence intensities at donor and acceptor emission wavelengths provides information on complexation via changes in donor-acceptor distance. The chapters by Valeur, Bouas-Laurent, and Krafft describe uses of the energy transfer mechanism. 

Huorescence resonance energy transfer (FRET) is a non-radiative, through-space excitation energy transfer process. The electronic excitation energy of a donor molecule is transferred to a ground-state acceptor through dipole-dipole interactions without involvement of a photon or molecular contact. Efficient FRET requires close proximity and suitable alignment between the transition dipoles of the donor and acceptor, as well as overlap between the donor emission spectrum and acceptor absorption spectrum. 

Ethylenediamine tetraacetic acid (or any of its conjugate acetate ions) Forster resonance energy transfer, a form of excitation energy transfer (sometimes referred to with the incorrect term fluorescence resonance energy transfer )... 

Through effective resonance-type energy transfer and co-excitation, the... 

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