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Resonance energy transfer polarization measurements

Cohen-Kashi, M., Namer, Y. and Deutsch, M. (2006). Fluorescence resonance energy transfer imaging via fluorescence polarization measurement. J. Biomed. Opt. 11, 34015. [Pg.517]

The following sections give an overview and some examples of the fluorescence detection methods currently used in HTS fluorescence intensity (FI) fluorescence polarization (FP) or fluorescence anisotropy (FA) fluorescence resonance energy transfer (FRET) Hfetime-based measurements (TRF and FLT) fluorescence corre-... [Pg.630]

Many types of assay are available to be used in HTS protocols to identify inhibitors of PPIs, but a competition assay, in which inhibition of complex formation is measured, is most common. Fluorescence polarization (FPA), fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays (ELISA), and other assay formats have been used. The interacting proteins can be used in their full-length forms though, more frequently only the interacting domains are employed, and if possible the excised interacting peptide is usually preferred. [Pg.9]


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Energy measurement

Energy resonant

Polarization energy

Polarization measurement

Polarized measurements

Resonance energy

Resonance measurements

Resonance transfer

Resonant polarization

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