Resonance assignments for

Fig. 2 Chemical shift perturbation and chemical shift mapping, (a) Portions of the [15N, 1H]-HSQC spectra of Bcf-xL recorded in absence (black) and in presence of each of the four molecules (in colors). Resonance assignments for amino acid residues that exhibit large shifts are reported, (b) Structure of Bc1-Xl in complex with the BH3 peptide from Bak (PDB code 1BXL) showing the chemical shift changes in Bcl-xL upon ligand binding (blue, large shits yellow, no shifts the Bak peptide is reported in cyan). Adapted from [48]...

On the other hand, resonance assignments for CP of threonine and serine, and C and Cy of hydroxy proline, were difficult to make, because of their proximity to carbohydrate carbon resonances. In most cases then, the resonances were assigned on the basis of the effects of pH on the chemical shifts of those resonances. It was shown that the chemical shifts for the carbohydrate carbon resonances were virtually unaffected (AS 0.4 p.p.m.) when going from the cationic state (pH 2) to the anionic state (pH 11) of the amino acid residues. The chemical shifts of C and CP of the amino acid residues, however, shifted considerably (up to 3.1 and 6.6 p.p.m. for C" and CP, respectively see Table VI). 

Table 3. Proton nuclear magnetic resonance assignments for spectrum of sodium valproate...

Fig. 21.4 Three-dimensional structure of the enzyme cytochrome P450 oxidoreductase. The cofactors FAD and FMN are depicted in light blue. Loop regions are represented by cylindrical rods (yellow) a-helices and /(-sheets (in white) are represented by ribbons and arrows, respectively. Resonance assignments for the residues located in the key loops regions [30] highlighted in purple and red were obtained with a 3D SEA-HNCA-TROSY experiment.

TABLE 7.6 hi and 13C resonance assignments for vigabatrin racemic mixture and (A) isomer... 

The proton magnetic resonance assignments for 241D have been presented (87). The optical rotation, is -1-39° (0.2, CHjOH). The structure of 241D has been confirmed by synthesis (M. W. Edwards, personal communication, 1990). None of the other amphibian piperidines has been isolated for further spectral analysis. However, Bohlmann bands in FTIR spectra will allow assignment of cis or trans configurations to such 2,6-disubstituted piperidines (see Section III,B). 

The protein chemistry, much of the spectroscopy and NMR resonance assignments for op repressor were carried out in the Arrowsmith laboratory with support from the NCI of Canada and the Human Frontier Science Program. We acknowledge our very fruitful collaboration with the laboratory of O. Jardetzky on the structure of the trp repressor complex. The development and implementation of the HNCA and isotope filtered pulse sequences was carried out in the laboratory of L.E. Kay with support from NSERC and NCI of Canada. T. Yamazaki acknowledges fellowsldp support from the Human Frontier Science Program. 

NCOCA- and NCACB-type experiments, such as those discussed in Section 2.6, were used to obtain an almost complete set of de novo resonance assignments for the SH3 domain of a-spectrin (62 residues, 7.2 kDa) under MAS conditions. " As discussed above, correlation of these ( N, C) chemical shift assignments with random coil values subsequently showed " that secondary chemical shifts provide a straightforward and powerful instrument with which to monitor secondary structure in solid-phase proteins under MAS conditions. Moreover, these resonance assignments represented the starting point for an investigation of the proton resonance frequencies and for the structural... 

Finally, we have obtained ( C, N) resonance assignments for the 38-amino acid polypeptide kaliotoxin (KTX), " a 38-residue peptide found in the venom of the scorpion Androctonus mauretanicus mauretanicus KTX has been shown to block voltage-dependent eukaryotic potassium (Kv) channels (e.g.. Shaker and Kvl.3) in high... 

Proton-decoupled, C-n.m.r.-spectral data and resonance assignments for D-gluconamides 6-10 are provided in Table III. These compounds... 

The assignments may have to be interchanged. Specific, resonance assignments for C-9 and C-10 were not made (taken from Ref. 68). 

Varga K, Aslimovska L, Watts A (2008) Advances towards resonance assignments for uniformly - C-13, N-15 enriched bacteriorhodopsin at 18.8 T in purple membranes. J Biomol NMR 41 1 ... 

Figure 19. Selected regions from the slice of the HCP, H(CA)P-CC-TOCSY and HCCH experiments, showing the resonance assignments for one of the P-containing chain-ends of the polymer formed of diphenylphosphinyl radical initiated polymerization of styrene. (Reproducedfrom reference 19. Copyright 2001 American Chemical Society.)...

Habenstein et al. have presented the de novo resonance assignments for the crystalline 33kDa C-terminal domain of the Ure2 prion using an... 

D-NMR methods have also been used to determine coupling constants and resonance assignments for an alternating copolymer of TFE and propylene 13. Research on an Aflas fluoroelastomer sample dissolved in THF-t/g was reported by Kuroki... 

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