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Resin-embedded sections protocol

If epoxy resin-embedded tissue is used, cut 2- jm-thick sections with a glass knife, mount on APES-coated slides, and dry as described in Note 2. Deplasticize the sections by immersing them in sodium eth(meth)oxide for 15 min (8). Wash the sections twice with equal parts of methanol (or IMS) and xylene, twice with methanol, for 3 min each, and rehydrate. Afterward, the same HIER and immuno-histochemical protocols are employed as in paraffin sections. [Pg.91]

See other pages where Resin-embedded sections protocol is mentioned: [Pg.104]    [Pg.92]    [Pg.248]    [Pg.248]    [Pg.53]    [Pg.286]    [Pg.87]    [Pg.183]    [Pg.183]    [Pg.43]    [Pg.375]   
See also in sourсe #XX -- [ Pg.2 , Pg.121 ]



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