Residue analysis aflatoxins

Table Aflatoxin Residue Analysis in Freeze-Dried Tissues ...

The analysis of food contaminants, in particular any toxic or biologically active residue, is important for public health or quality control reasons.19 Examples are mycotoxins (aflatoxins) and pesticide and drug residues. Sample preparation is typically elaborate and might involve deproteinization, solvent extraction, and clean-up via solid-phase extraction (SPE).The use of highly sensitive and specific LC/MS/MS is increasing and has simplified some of the sample preparation procedures. 

The evaluation of a number of immunoassay diagnostic kits was undertaken to determine their usefulness in a regulatory analytical laboratory environment in the food, feed and pesticide areas. Four rapid enzyme immunoassay tests for the detection of aflatoxin residues at the 20 ppb level in animal feeds were compared to the official HPLC procedure. In the pesticide area, a commercial pentachlorophenol competitive inhibition assay for residues in water was investigated as to its applicability to poultry and pork liver matrices. In addition, an ELISA screening procedure for the herbicide fusilade was developed. Modifications were incorporated into the rapid immunoband 1-2 Test procedure for the detection of motile Salmonella in various food and animal feed products resulting in quicker analysis than the standard culture method. Also, a comparative evaluation of a Quik-Card Test for sulphamethazine drug residues in pork urine, liver and muscle tissue, is described. 

AgricultureCanada has an ongoing program of testing feeds from the domestic market and export certification for aflatoxin 1, B2, G1, G2) residues using an HPLP technique sensitive at the 2.5 ppb level for each aflatoxin with results being reported as "total" aflatoxin. To help increase sample throughput and reduce analysis time, four commercially available enzyme immunoassay tests for aflatoxin residues were evaluated. 

In this scene, HPLC is a paramount technique in the modern age of food analysis with hundreds of applications [3], some of which include the detection of aflatoxins, amino acid analysis, vitamins separation, profiling of various food components, analysis of colorants and their residues, or the determination of the sugars content. 

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