Reporter genes Renilla luciferase

While the applications of SELP-mediated controlled gene delivery are numerous, we have focused our efforts on controlled delivery to solid tumors. Our initial studies in this arena involved the intratumoral delivery of a reporter plasmid Renilla luciferase) to solid tumors in a murine (athymic nu/nu) model of human breast cancer (MDA-MB-435 cell line) (50). Delivery of the Renilla luciferase plasmid from SELP-47K matrices resulted in significantly enhanced tumor transfection for up to 21 days compared to naked DNA (Fig. 5). In particular, delivery of the plasmid from matrices containing 4 or 8 wt.% polymer resulted in enhanced transfection... 

Glow luminescence techniques have been used extensively with luciferases as reporter genes in cell-based assays. An overview of such assays is given in Section 10.3.2 Reporter Assays below. Luciferases are enzymes which catalyze bio-luminescent reactions. Two forms are used as reporters, one originating from the firefly (firefly luciferase) and the other from Rmilla (Renilla luciferase). Due to their different origins, the enzyme structures and their respective substrates are quite different While Rmilla luciferase catalyzes the oxidation of coelenterazine, the substrate of firefly luciferase is the beetle luciferin, which is oxidized in the presence of ATP and Mg as depicted in Fig. 17. 

Fig. 2 Schematic of liposome loaded with a tri-fusion reporter gene (TF). (A) Schematic representation of the tri-fusion reporter gene (TF) containing Renila luciferase (Rluc), red fluorescent protein (RFP) and HSV-ttk driven by a CMV promoter. (B) Schematic representation of liposome nanoparticle and liposome-DNA coupling reaction. Therapeutic liposomes were loaded with Rluc-RFP-ttk gene. (C) The expression of TF transgene was tracked and analyzed by imaging and immunofluorescence. Imaging of Renilla luciferase intensity from one representative BALB/c mouse [39].

Bhaumik S, Gambhir SS. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proc Natl Acad Sci USA 2002 99 377-382. 

In another report. Park et al. have successfully fabricated a cell-free protein-producing gel that can produce proteins 300 times more efficiently than existing solution processes [87]. Ligation of the gene of interest, in this case the renilla luciferase gene, with X-DNA connectors by DNA Hgase yielded a protein-producing... 

Srikantha T, Klapach A, Lorenz WW, Tsai LK, LaughUn LA, Gorman JA, Soil DR. The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. J Bacteriol 1996 178 121-129. 

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