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Replication mapping

Diagram illustrating the replication mapping experiment. To a single-stranded, platinated template is annealed a short primer for DNA synthesis using DNA polymerase I (Klenow fragment) and radiolabeled nucleotides. Sites of platinum binding are revealed as bands on gel electrophoresis where chain termination occurs (see text for details). [Pg.552]

Figure 6. Schematic diagram showing the replication mapping methodology. A platinum complex is added to DNA to form a cross-link and the site of platination is revealed by primer annealing, DNA polymerase chain extension, and gel electrophoresis analytical steps (see Ref. 96 for details). Figure 6. Schematic diagram showing the replication mapping methodology. A platinum complex is added to DNA to form a cross-link and the site of platination is revealed by primer annealing, DNA polymerase chain extension, and gel electrophoresis analytical steps (see Ref. 96 for details).
As seen in the genetic map, the genes after gene 1.1, transcribed by the T7 RNA polymerase, code for proteins that are involved in T7 DNA synthesis, the formation of virus coat proteins, and assembly. Three classes of T7 proteins are formed class I, made 4-8 minutes after infection, which use the cell RNA polymerase class II, made 6-15 minutes after infection, which are made from T7 RNA polymerase and are involved in DNA metabolism class III, made from 6 minutes to lysis, which are transcribed by T7 RNA polymerase and which code for phage assembly and coat protein. This sort of sequential pattern, commonly seen in many large double-stranded DNA phages, results in an efficient channeling of host resources, first toward DNA metabolism and replication, then on to formation of virus particles and release of virus by cell lysis. [Pg.142]

Figure 5.19 Simplified genetic map of T4. Late genes with morphogenetic functions (coat proteins and assembly), and genes with functions in DNA replication are identified. Note that although the genetic map is represented as a circle, the DNA itself is actually linear. Figure 5.19 Simplified genetic map of T4. Late genes with morphogenetic functions (coat proteins and assembly), and genes with functions in DNA replication are identified. Note that although the genetic map is represented as a circle, the DNA itself is actually linear.
Bucholz KK, Li T-K, Hesselbrock V, Crowe R, Schuckit M, Porjesz B, Begleiter H, Reich T. Alcoholism susceptibility loci confirmation studies in a replicate sample and further mapping. Alcohol Clin Exp Res 2000 24 933-945. [Pg.439]

The summary suggests that a number of psychopathology taxa have been identified to date and some forms of psychopathology were found to be continuous. However, support for many of these taxa and continua is rather weak and replications are desperately needed. In fact, at this point we cannot say that the taxonic status of any of the DSM disorders is known with certainty. Constructs where the structure has been consistently supported (e.g., schizotypy or depression severity) do not map clearly on the DSM. Only a handful of taxometric studies have directly evaluated DSM diagnoses, and multiple replications are needed before any firm conclusions can be drawn. [Pg.174]

L. T. Vassilev, W. C. Burhans, and M. L. DePamphilis, Mapping an origin of DNA replication at a single-copy locus in exponentially proliferating mammalian cells. Mol. Cell. Biol. 10,4685-4689 (1990). [Pg.251]

CDP-choline Choline donor Endogenous nucleotide APOE Retrospective Prospective Genotype dependent Genotype dependent Cognition Brain blood flow Brain mapping Bio-chemistry Replicated in two studies... [Pg.302]

It is suggested that PPARa activation may lead to DNA replication, cell growth, and inhibition of apoptosis via tumor necrosis factor a (TNF-a). Alternatively or additionally, the oxidative stress could activate the MAP kinase pathway, which will also influence cell growth (Fig. 7.11), but this might be independent of PPARa activation. However, the role of these events is still unclear. [Pg.307]


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See also in sourсe #XX -- [ Pg.552 ]

See also in sourсe #XX -- [ Pg.491 , Pg.492 ]




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