Replication factories

Precision Repeatability Ruggedness Reproducibility Sample prep replication Injection replication Factorial designs Intra-laboratory study Inter-laboratory study... 

Bacterial Replication Is Organized in Membrane-Bound Replication Factories... 

RGURE 25-18 Chromosome partitioning in bacteria, (a) All replication is carried out at a central replication factory that includes two complete replication forks, (b) The two replicated copies of the bacterial chromosome are extruded from the replication factory into the two halves of the cell, possibly with each newly synthesized origin bound separately to different points on the plasma membrane. Sequestering the two chromosome copies in separate ceil halves facilitates their proper segregation at ceil division. 

Replication of the E. coli chromosome involves many enzymes and protein factors organized in replication factories, in which template DNA is spooled through two replisomes tethered to the bacterial plasma membrane. 

Report describing the replication factory model for bacterial DNA replication. 

Adds a section on the "replication factories" of bacterial DNA Includes latest perspectives on DNA recombination and repair... 

Compound recovery data for duplicate runs differed by 2-15, depending on the compound. Half-normal probability plot analysis of the new data for the anomalous compounds indicated none of the distortion encountered earlier. Results for acetone and tetrachloroethylene now indicated only random variation with no significant outliers. Results for 2,4-dichlorophenol and 2,5-dichlorophenol indicated a significant pH effect. A significant interaction effect (AB) was detected between variables pH and primary column type for the dichlorophenols and also for methyl isobutyl ketone. This interaction effect indicates that at approximately low pH (pH 2), compound recoveries for dichlorophenols will be greater when a C18 phase is used as the primary column. The half-normal plot for 2,5-dichlorophenol is shown in Figure 10. In examining data for all the compounds from the 23 replicate factorials, this interaction consistently appears for phenolic compounds. 

Nair, V. N. and Pregibon, D. (1988). Analyzing dispersion effects from replicated factorial experiments. Technometrics, 30, 247-257. 

FIGURE 25-18 Chromosome partitioning in bacteria, (a) All replication is carried out at a central replication factory that... 

Aparicio T, Megias D, Mendez J. Visualization of the MCM DNA helicase at replication factories before the onset of DNA synthesis. Chromosoma. 2012 121 499-507. 

Figure 10.1 below is a piece of artwork that was reproduced from the NASA study. The description below the picture describes one of the features as a self-replicating factory In the lower left corner, a lunar manufacturing facility rises from the surface of the Moon. Someday, such a factory might replicate itself, or at... 

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