Released oligosaccharides purification

To allow all culture productiou to be coutrolled, a method for rapid analysis is required. Prior to development of an LC-MS method, the analysis was both complex and time-consuming, involving the purification of a relatively large amount of the antibody using affinity chromatography, enzymatic release, and subsequent derivatizafion of the oligosaccharides and their analysis by using capillary electrophoresis. 

After the release of the oligosaccharides, they must be purified by a variety of methods before structural analyses can be undertaken. Ion-ex-change chromatography, gel-filtration chromatography, or some type of electrophoresis is usually used for the purification. At this point, structural analyses may begin. 

SPOS also benefited from other technical improvements such as the on-resin analytical methods [112], the capture-release purification technique [113], and hydrophobically assisted switching phase [114], Recently, chiral auxiliary-mediated 1,2-n.v-glycosylations were introduced to SPOS, allowing for the efficient and stereoselective assembly of oligosaccharides [115] (see Chapter 7). 

The isolation of products is sometimes a tedious and time-consuming procedure. Especially in the area of combinatorial synthesis of oligosaccharides either in solution or on solid-phase, a simple and effective isolation method is necessary because many compounds are handled at the same time. For instance, chromatography is a quite popular method for isolation of the products of organic synthesis. However, it is somewhat tedious and consumes a considerable amount of solvents, and optimization of the separation conditions sometimes requires much time. Therefore, techniques for pol)mier-supported purification such as catch-and-release ... 

Release of oligosaccharides from the glycopeptides and purification of the oligosaccharides. 

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