Rehydratable gels studies

Rehydratable gels were obtained from Micro-Map, Inc., Boca Rotan, FL, at both 3 per cent T, 3.5 per cent C and 5 per cent, T 3.5 per cent C. (T refers to the per cent of monomer present, while C is defined as the per cent of cross-linker relative to T. The per cent of the gel is the total of these two values) For comparative studies conventional gels were prepared at the same acrylamide concentration from the same bottle of two times recrystallized acrylamide (Serva). Acrylamide was recrystallized also from chloroform as previously described [2]. These gels were 250 or 375 1 thick and were bonded to silanized glass plates, or covalently bonded to GelBond Pag 7 mil mylar sheets (Marine Colloids, Rockland ME ) using previously published methods 118-191 and were randomized between the two laboratories. Single lots of ampholytes from various manufacturers were used for the comparative studies in each laboratory to eliminate lot variation. 

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